BIOGENIC AMINES CONTENT OF POULTRY BREAST STORED UNDER DIFFERENT CONDITIONS AT + 4°C FROM TWO DIFFERENT INDUSTRIAL GENOTYPE CHICKENS

In this experimental work, forty breast chickens of two different industrial genotype, were analysed at 4, 8, 11 and 15 days from slaughtering. All breast chickens were divided in two groups. A group was stored at 4°C, and the other was stored at 4°C in sterilised water added with antibiotic (400 mg/l Enrofloxacin). BAs quantitative determination was carried out by means of HPLC, with spectrophotometric-UV detection. The amines were extracted in HClO4 and derivatised by dansylchloride. In the samples stored in sterile water added with antibiotic, no bacterial growth was observed until the end of the experimental period. Significant differences in BAs accumulation were observed between the two samples groups, while BAs content was different during time. In fact, in samples stored in sterilised water added with the antibiotic, the presence of some BAs, such as spermine and spermidine, was revealed, probably due to the action of tissue enzymes.