Yeasts isolated from Italian beverages and foods (wine and cheeses) were identi fi ed as Saccharomycescerevisiae and Debaryomyces hansenii by sequencing the D1/D2 domain of the 26S rRNA gene and differenti-ated, at strain level, by microsatellite PCR fi ngerprinting and RAPD-PCR.All the strains showed antioxidant activity, as demonstrated by their ability to scavenge the free radicaldiphenyl-1-picrylhydrazyl (DPPH). Furthermore, tested strains revealed high in vitro inhibitory activity againsttwo model genotoxins, 4-nitroquinoline-1-oxide (4-NQO) and N-methyl-N′-nitro-N-nitrosoguanidine (MNNG),as showed by short-term methods with different target cells: SOS-Chromotest with Escherichia coli PQ37 andComet assay with HT-29 enterocytes. High inhibitory activity towards 4-NQO was associated with cell viability,while heat-inactivated cells showed a reduced antigenotoxic capability. Surprisingly, high inhibition of MNNGgenotoxicity was observed even with heat-treated cells. Moreover, the strains able to inhibit the genotoxins in-duced some changes in the spectroscopic properties of the original compound. This result perfectly agrees withthe information obtained by the two bioassays.Interestingly, strains characterized for antioxidant and antigenotoxic properties, also presented acid-bile toler-ance, indicating that food autochthonous yeasts could be expected to reach gut in viable form and thus preventgenotoxin DNA damage in situ.[...]

Food borne yeasts as DNA-bioprotective agents against model genotoxins

TOFALO, ROSANNA;SCHIRONE, MARIA;CORSETTI, Aldo;SUZZI, Giovanna;
2012-01-01

Abstract

Yeasts isolated from Italian beverages and foods (wine and cheeses) were identi fi ed as Saccharomycescerevisiae and Debaryomyces hansenii by sequencing the D1/D2 domain of the 26S rRNA gene and differenti-ated, at strain level, by microsatellite PCR fi ngerprinting and RAPD-PCR.All the strains showed antioxidant activity, as demonstrated by their ability to scavenge the free radicaldiphenyl-1-picrylhydrazyl (DPPH). Furthermore, tested strains revealed high in vitro inhibitory activity againsttwo model genotoxins, 4-nitroquinoline-1-oxide (4-NQO) and N-methyl-N′-nitro-N-nitrosoguanidine (MNNG),as showed by short-term methods with different target cells: SOS-Chromotest with Escherichia coli PQ37 andComet assay with HT-29 enterocytes. High inhibitory activity towards 4-NQO was associated with cell viability,while heat-inactivated cells showed a reduced antigenotoxic capability. Surprisingly, high inhibition of MNNGgenotoxicity was observed even with heat-treated cells. Moreover, the strains able to inhibit the genotoxins in-duced some changes in the spectroscopic properties of the original compound. This result perfectly agrees withthe information obtained by the two bioassays.Interestingly, strains characterized for antioxidant and antigenotoxic properties, also presented acid-bile toler-ance, indicating that food autochthonous yeasts could be expected to reach gut in viable form and thus preventgenotoxin DNA damage in situ.[...]
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11575/16603
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