In this study, the development and validation of a multiresidue method for the detection of 11 quinolones (marbofloxacin, norfloxacin, ciprofloxacin, danofloxacin, lomefloxacin, enrofloxacin, sarafloxacin, difloxacin, oxolinic acid, nalidixic acid, flumequine) in muscle and eggs were reported. The method involved an extraction with a methanol/metaphosphoric acid mixture and a clean up by Oasis hydrophilic-lipophilic balance (HLB) cartridge. The validation was performed according to the Commission Decision 2002/657/EC. Linearity, specificity, decision limit (CCα), detection capability (CCβ), recovery, precision (repeatability and within-laboratory reproducibility), and ruggedness were determined. Depending on the analytes, CCα and CCβ ranged from 113 to 234Â μg/kg and from 126 to 282Â μg/kg in muscle samples, whereas in eggs, these parameters were between 5.6 and 7.4Â μg/kg and between 6.1 and 9.8Â μg/kg, respectively. In both the examined matrices, the recovery values were always higher than 90Â % and precision, calculated as relative standard deviation, was equal to or lower than 16Â % for repeatability and 23Â % for within-laboratory reproducibility. The described method can be considered adequate for the simultaneous determination and quantification of quinolones in the tested food matrices.
|Titolo:||Development and Validation of a Method for the Determination of Quinolones in Muscle and Eggs by Liquid Chromatography-Tandem Mass Spectrometry|
|Autori interni:||ANNUNZIATA, LOREDANA|
|Data di pubblicazione:||2016|
|Rivista:||FOOD ANALYTICAL METHODS|
|Appare nelle tipologie:||1.1 Articolo in rivista|