Listeriosis is a foodborne disease caused by Listeria monocytogenes, with a significant morbidity and mortality rate in people with a weakened immune system, principally pregnant women, newborn, young, and elderly. Many outbreaks were associated with the consumption of raw milk and dairy products (principally white or soft cheeses), poultry and processed meat, seafood, and ready to eat foods (salads, raw vegetables, etc.). Severe clinical manifestations varied from self-limited gastroenteritis to septic abortion, encephalitis, bacteremia, and meningitis. Listeria monocytogenes can tolerate, grow and/or multiply over a wide range of environmental conditions that can be adopted to control food contamination (Chafsey et al., 2022), such as low pH, refrigeration temperatures, low water activity, and high salt concentrations. The current study evaluated the exposure effect of two L. monocytogenes 1/2a strains isolated from meat products at four different growth conditions: A (control): T 37°C, pH 7.0, NaCl 0.5%; B: T 37°C, pH 5.5, NaCl 7%; C: T 12°C, pH 7.0, NaCl 0.5%; D: T 12°C, pH 5.5, NaCl 7%. A proteomic approach was applied to evaluate which proteins were produced by the strains when exposed at different temperatures, pH, and salt concentrations. The whole proteome was analyzed resolving the protein extracts by 1D-electrophoresis. A shotgun proteomics analysis was performed to identify proteins of interest. The entire gel lanes were digested and analyzed by nLC-ESI-MS/MS technique. Several different targets were identified and characterized examining the corresponding gene list by ShinyGO tool (Ge et al., 2020). The analysis of the whole proteome profiles highlighted a significant inter-strain variation in the proteins produced. Most of the proteins identified were produced in growth conditions and were involved in basal cell metabolism. Differently, some proteins defined as unique for each condition are correlated with the pathway of pathogenicity and stress response. The proteomic data obtained in this study will be the base for further research to deeply investigate L. monocytogenes pathogenicity and stress adaptation, in order to prevent or mitigate future outbreaks.
A proteomic approach to analyze the response of Listeria monocytogenes to different environmental conditions
D'Onofrio F.;Visciano P.;Paparella A.;Schirone M.
2022-01-01
Abstract
Listeriosis is a foodborne disease caused by Listeria monocytogenes, with a significant morbidity and mortality rate in people with a weakened immune system, principally pregnant women, newborn, young, and elderly. Many outbreaks were associated with the consumption of raw milk and dairy products (principally white or soft cheeses), poultry and processed meat, seafood, and ready to eat foods (salads, raw vegetables, etc.). Severe clinical manifestations varied from self-limited gastroenteritis to septic abortion, encephalitis, bacteremia, and meningitis. Listeria monocytogenes can tolerate, grow and/or multiply over a wide range of environmental conditions that can be adopted to control food contamination (Chafsey et al., 2022), such as low pH, refrigeration temperatures, low water activity, and high salt concentrations. The current study evaluated the exposure effect of two L. monocytogenes 1/2a strains isolated from meat products at four different growth conditions: A (control): T 37°C, pH 7.0, NaCl 0.5%; B: T 37°C, pH 5.5, NaCl 7%; C: T 12°C, pH 7.0, NaCl 0.5%; D: T 12°C, pH 5.5, NaCl 7%. A proteomic approach was applied to evaluate which proteins were produced by the strains when exposed at different temperatures, pH, and salt concentrations. The whole proteome was analyzed resolving the protein extracts by 1D-electrophoresis. A shotgun proteomics analysis was performed to identify proteins of interest. The entire gel lanes were digested and analyzed by nLC-ESI-MS/MS technique. Several different targets were identified and characterized examining the corresponding gene list by ShinyGO tool (Ge et al., 2020). The analysis of the whole proteome profiles highlighted a significant inter-strain variation in the proteins produced. Most of the proteins identified were produced in growth conditions and were involved in basal cell metabolism. Differently, some proteins defined as unique for each condition are correlated with the pathway of pathogenicity and stress response. The proteomic data obtained in this study will be the base for further research to deeply investigate L. monocytogenes pathogenicity and stress adaptation, in order to prevent or mitigate future outbreaks.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
