Autism spectrum disorders (ASDs) involve central nervous system (CNS), immune and gastrointestinal (GI) dysfunctions, linked via the gut-brain axis . In the Neuroligin3 R451C knock-in (KI) monogenic mouse model of autism 5-weeks of a food-borne Lactiplantibacillus (Lpb.) plantarum supplementation improved impaired social behavior and restored synaptic and gut markers. We assessed the effectiveness of Lpb. plantarum on impaired social behavior of KI mice through the three chambers test, observing a rescue to WT mice values on sociability index. We analyzed both excitatory and inhibitory synaptic proteins levels (PSD-95, Gephyrin), finding a rescue in their expression to the WT values after Lpb. plantarum intake. We also analyzed gut microbiota composition, finding an increase in gut-supporter taxa in KI mice fed with Lpb. plantarum. Finally, we assessed intestinal permeability by analyzing through rt-PCR the gene expression of tight junctions (Claudin-1, Claudin-3 and Zonulin-1), observing a beneficial effect of Lpb. plantarum administration in KI mice, in colon, cecum and ileum gut regions. These findings support microbiota-targeted therapies for ASDs.
THE GUT-BRAIN AXIS AS A THERAPEUTIC TARGET IN NEUROLIGIN3 R451C KNOCK-IN MOUSE MODEL OF AUTISM: ROLE OF LACTIPLANTIBACILLUS (LPB.) PLANTARUM
G. Sabatini;R. Prete;A. Corsetti;N. Battista;
2025-01-01
Abstract
Autism spectrum disorders (ASDs) involve central nervous system (CNS), immune and gastrointestinal (GI) dysfunctions, linked via the gut-brain axis . In the Neuroligin3 R451C knock-in (KI) monogenic mouse model of autism 5-weeks of a food-borne Lactiplantibacillus (Lpb.) plantarum supplementation improved impaired social behavior and restored synaptic and gut markers. We assessed the effectiveness of Lpb. plantarum on impaired social behavior of KI mice through the three chambers test, observing a rescue to WT mice values on sociability index. We analyzed both excitatory and inhibitory synaptic proteins levels (PSD-95, Gephyrin), finding a rescue in their expression to the WT values after Lpb. plantarum intake. We also analyzed gut microbiota composition, finding an increase in gut-supporter taxa in KI mice fed with Lpb. plantarum. Finally, we assessed intestinal permeability by analyzing through rt-PCR the gene expression of tight junctions (Claudin-1, Claudin-3 and Zonulin-1), observing a beneficial effect of Lpb. plantarum administration in KI mice, in colon, cecum and ileum gut regions. These findings support microbiota-targeted therapies for ASDs.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
