An ecological fact is that a developing zebrafish juvenile needs to feed actively to support its growth and development, so an ecotoxicological assay of feed samples is required to accommodate this key event to allow for proper extrapolation towards the population metrics. For this, the present study, was conducted to evaluate the ecotoxic effect of : 1) different market dry feed samples used for feeding zebrafish, 2) artemia (zebrafih live feed) 3) water (zebrafish are normally living in the circulating water flow system ) via Microtox® analyser. Our interest is to check the water and feed quality used during the zebrafish breeding. For this purpose, dry feed samples with trade names such as ZM-000, Gemma-75, ZM-100, Gemma-150, ZM-200, Gemma-300, artemia and water were evaluate for ecotoxic effect in 05, 15 and 20 minutes using bioluminescence technology via Microtox® M500 analyzer. It has been shown that all samples have ecotoxic effect at dilution 3rd and 4th. While ZM-000, Gemma-75 and ZM-100 have negative, negligible and negative ecotoxic effect respectively. The dilution 2nd has zero, zero and slight ecotoxic effect for all samples. It has been revealed that all dilution of ZM-200 have ecotoxic effect. While ZM-150, and Gemma-300 have significant ecotoxic effect at dilution 4th while for dilution 1st, 2nd and 3rd has zero, slight ecotoxic effect for ZM-150 and Gemma-300 samples. All dilution of artemia, water-1 and water have no ecotoxic effect while just dilution 4th of artemia has slight effect. However, artemia didn‘t showed any ecotoxic effect as it was not possible to dissolve so it would be better to repeat the test using hatched live artemia. It could be concluded that both market dry feed samples have shown several ecotoxic effects may be due to the presence of endrocrine disruptors or other environmental chemicals in the feed composition. So it is further recommended that dry feed samples which are used for zebrafish feeding should be evalauted for chemcials presence. The water have shown negative ecotoxic effect. This result could be due to the inability of the chemicals present in the feed to dissolve in the water or because these are removed by the circulating water flow system.
Evaluation of ecotoxic effect of zebrafish dry feed and water via Microtox® M500 Analyser
NIAZ, KAMAL;Giovanni Angelozzi;Monia Perugini
2018-01-01
Abstract
An ecological fact is that a developing zebrafish juvenile needs to feed actively to support its growth and development, so an ecotoxicological assay of feed samples is required to accommodate this key event to allow for proper extrapolation towards the population metrics. For this, the present study, was conducted to evaluate the ecotoxic effect of : 1) different market dry feed samples used for feeding zebrafish, 2) artemia (zebrafih live feed) 3) water (zebrafish are normally living in the circulating water flow system ) via Microtox® analyser. Our interest is to check the water and feed quality used during the zebrafish breeding. For this purpose, dry feed samples with trade names such as ZM-000, Gemma-75, ZM-100, Gemma-150, ZM-200, Gemma-300, artemia and water were evaluate for ecotoxic effect in 05, 15 and 20 minutes using bioluminescence technology via Microtox® M500 analyzer. It has been shown that all samples have ecotoxic effect at dilution 3rd and 4th. While ZM-000, Gemma-75 and ZM-100 have negative, negligible and negative ecotoxic effect respectively. The dilution 2nd has zero, zero and slight ecotoxic effect for all samples. It has been revealed that all dilution of ZM-200 have ecotoxic effect. While ZM-150, and Gemma-300 have significant ecotoxic effect at dilution 4th while for dilution 1st, 2nd and 3rd has zero, slight ecotoxic effect for ZM-150 and Gemma-300 samples. All dilution of artemia, water-1 and water have no ecotoxic effect while just dilution 4th of artemia has slight effect. However, artemia didn‘t showed any ecotoxic effect as it was not possible to dissolve so it would be better to repeat the test using hatched live artemia. It could be concluded that both market dry feed samples have shown several ecotoxic effects may be due to the presence of endrocrine disruptors or other environmental chemicals in the feed composition. So it is further recommended that dry feed samples which are used for zebrafish feeding should be evalauted for chemcials presence. The water have shown negative ecotoxic effect. This result could be due to the inability of the chemicals present in the feed to dissolve in the water or because these are removed by the circulating water flow system.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
