Objectives This study evaluated synovial and systemic plasma pharmacokinetic variables of marbofloxacin after a single intravenous regional limb perfusion (IVRLP) performed using two different tourniquets in clinically healthy, standing, non-sedated dairy cows. The authors hypothesised that the type of tourniquet used for IVRLP would influence the synovial fluid concentration of marbofloxacin in the perfused distal limb. Design The study had a randomised parallel-group design. Methods Ten adult dairy cows were included. Unilateral hindlimb IVRLP through the dorsal common digital III vein was performed in two groups of five cows (group 1: wide rubber tourniquet; group 2: manual pneumatic tourniquet) using 0.67 mg/kg of marbofloxacin. The tourniquet was applied proximal to the tarsus and maintained for 30 minutes. Samples of jugular blood and synovial fluid from the tibiotarsal joints of the perfused limb were obtained before and at intervals after IVRLP. All samples were analysed for drug concentrations using liquid chromatography tandem mass spectrometry. Pharmacokinetic parameters were determined to establish the influence of tourniquet types. Differences were considered significant at P≤0.05. Results Noadverseeffectsfromtheprocedureor marbo oxacin were observed in any animal. Signi cant differences in synovial concentrations and pharmacokinetic parameters were measured. The mean ± sd areas under the concentration versus time curve from time 0 to 24hours were 178.98±58.08μg hour/ml for group 2 and 21.11±9.93 μg hour/ml for group 1. The mean ± sdmaximum marbo oxacin concentrations were 75.50±10.19μg/ml for group 2 and 6.35±1.47μg/ml for group 1. Conclusions PerformingIVRLPusingthedorsalcommon digital III vein and a manual pneumatic tourniquet set at 300 mmHg above the tarsus in standing cows resulted in signi cantly higher marbo oxacin concentrations in the tibiotarsal joint compared with those with the wide rubber tourniquet. Trial registration Local ethical committee (number 41/2012/CEISA).
Pharmacokinetics of marbofloxacin administered via intravenous regional limb perfusion in dairy cows: evaluation of two different tourniquets
CELANI, GIANLUCA;TULINI, SERENA MARIA RITA;ZEZZA, DANIELA;SERGI, Manuel;VARASANO, Vincenzo;COMPAGNONE, DARIO;AMORENA, Michele;PETRIZZI, Lucio
2017-01-01
Abstract
Objectives This study evaluated synovial and systemic plasma pharmacokinetic variables of marbofloxacin after a single intravenous regional limb perfusion (IVRLP) performed using two different tourniquets in clinically healthy, standing, non-sedated dairy cows. The authors hypothesised that the type of tourniquet used for IVRLP would influence the synovial fluid concentration of marbofloxacin in the perfused distal limb. Design The study had a randomised parallel-group design. Methods Ten adult dairy cows were included. Unilateral hindlimb IVRLP through the dorsal common digital III vein was performed in two groups of five cows (group 1: wide rubber tourniquet; group 2: manual pneumatic tourniquet) using 0.67 mg/kg of marbofloxacin. The tourniquet was applied proximal to the tarsus and maintained for 30 minutes. Samples of jugular blood and synovial fluid from the tibiotarsal joints of the perfused limb were obtained before and at intervals after IVRLP. All samples were analysed for drug concentrations using liquid chromatography tandem mass spectrometry. Pharmacokinetic parameters were determined to establish the influence of tourniquet types. Differences were considered significant at P≤0.05. Results Noadverseeffectsfromtheprocedureor marbo oxacin were observed in any animal. Signi cant differences in synovial concentrations and pharmacokinetic parameters were measured. The mean ± sd areas under the concentration versus time curve from time 0 to 24hours were 178.98±58.08μg hour/ml for group 2 and 21.11±9.93 μg hour/ml for group 1. The mean ± sdmaximum marbo oxacin concentrations were 75.50±10.19μg/ml for group 2 and 6.35±1.47μg/ml for group 1. Conclusions PerformingIVRLPusingthedorsalcommon digital III vein and a manual pneumatic tourniquet set at 300 mmHg above the tarsus in standing cows resulted in signi cantly higher marbo oxacin concentrations in the tibiotarsal joint compared with those with the wide rubber tourniquet. Trial registration Local ethical committee (number 41/2012/CEISA).I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
