An amperometric enzyme electrode for the determination of aspartame was developed by covalent immobilization of alcohol oxidase and a-chymotrypsin, A platinum based hydrogen peroxide electrode was used as the detector, Excellent sensitivity was obtained using batch, flow-through and flow injection methods with detection limits of 2 x 10(-7), 4 x 10(-7) and 10(-6) mol l(-1), respectively, Different strategies for eliminating interfering compounds, including the introduction of an additional alcohol oxidase-catalase membrane and signal subtraction using an alcohol electrode, were employed, A recovery study on seven food samples was carried out and the results were satisfactory.[...]
Amperometric bienzymic sensor for aspartame
COMPAGNONE, DARIO;
1997-01-01
Abstract
An amperometric enzyme electrode for the determination of aspartame was developed by covalent immobilization of alcohol oxidase and a-chymotrypsin, A platinum based hydrogen peroxide electrode was used as the detector, Excellent sensitivity was obtained using batch, flow-through and flow injection methods with detection limits of 2 x 10(-7), 4 x 10(-7) and 10(-6) mol l(-1), respectively, Different strategies for eliminating interfering compounds, including the introduction of an additional alcohol oxidase-catalase membrane and signal subtraction using an alcohol electrode, were employed, A recovery study on seven food samples was carried out and the results were satisfactory.[...]I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
