The enzyme polyamine oxidase from maize has been purified and covalently immobilized onto polymeric supports to assemble a polyamine electrochemical biosensor. The enzyme has been used in conjunction with either an O-2 or a H2O2 electrode. The analytical behaviour of this enzyme electrode has been studied with respect to sensitivity, selectivity, optimum pH and buffer, response time and lifetime. Recovery studies performed on urine samples demonstrated that the polyamine biosensor developed can be successfully used to measure polyamines in biological fluids.[...]
Electrochemical probe for polyamines detection in biological fluids
COMPAGNONE, DARIO;
1998-01-01
Abstract
The enzyme polyamine oxidase from maize has been purified and covalently immobilized onto polymeric supports to assemble a polyamine electrochemical biosensor. The enzyme has been used in conjunction with either an O-2 or a H2O2 electrode. The analytical behaviour of this enzyme electrode has been studied with respect to sensitivity, selectivity, optimum pH and buffer, response time and lifetime. Recovery studies performed on urine samples demonstrated that the polyamine biosensor developed can be successfully used to measure polyamines in biological fluids.[...]File in questo prodotto:
Non ci sono file associati a questo prodotto.
I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
