Human 5-Lipoxygenase (5-LOX) is implied in the biosynthesis of leukotrienes, lipid mediators of inflammation. We performed a combined SAXS and SE-HPLC study to determine the oligomerization state of wild-type human 5-LOX in solution and possible differences with the crystal structure of the published stable 5-LOX mutant. Our results show that 5-LOX consists mainly of two dimeric species differing for their hydrodynamic volume. The question is open on possible effectors that might modulate monomer/monomer interactions and monomer/dimer equilibrium.
Three-dimensional structure of human 5-LOX in solution: new insights from SAXS analysis
SABATUCCI, Annalaura;Angelucci, C. B.;SIMONETTI, MONICA;DAINESE, Enrico
2015-01-01
Abstract
Human 5-Lipoxygenase (5-LOX) is implied in the biosynthesis of leukotrienes, lipid mediators of inflammation. We performed a combined SAXS and SE-HPLC study to determine the oligomerization state of wild-type human 5-LOX in solution and possible differences with the crystal structure of the published stable 5-LOX mutant. Our results show that 5-LOX consists mainly of two dimeric species differing for their hydrodynamic volume. The question is open on possible effectors that might modulate monomer/monomer interactions and monomer/dimer equilibrium.File in questo prodotto:
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