Canine spirocercosis is a life-threatening parasitosis caused by Spirocerca lupi (Nematoda, Spirurida) that is presently emergingin several countries. This study characterised an informative region within the mitochondrial (mtDNA) gene encoding for thecytochrome c oxidase subunit 1 (cox1) of S. lupi by Polymerase Chain Reaction (PCR)-coupled sequencing. Specimens from fivedifferent countries in Europe, Asia and Africa were examined and two different sequence variants of cox1 (i.e. haplotypes) weredetermined, displaying nucleotidic variation at 6 of 689 positions. All of these positions were invariable among all the parasiteindividuals from Europe (haplotype 1) and among the African and Asian individuals (haplotype 2), but differed between Europe andAsia/Africa. The S. lupi cox1 sequences were consistent with those of other common Spirurida previously reported at bothnucleotidic and phylogenetic levels. This study provides molecular information essential for identification of the nematode,irrespective of its life cycle stage. Crucial implications for the specific molecular diagnosis of clinical spirocercosis andinvestigation of the evolution, population genetics, ecology and epidemiology of S. lupi are discussed.[...]
Mitochondrial cytochrome c oxidase subunit 1 (cox1) gene sequence of Spirocerca lupi (Nematoda, Spirurida) from different geographical areas: avenues for potential implications
TRAVERSA, Donato;
2007-01-01
Abstract
Canine spirocercosis is a life-threatening parasitosis caused by Spirocerca lupi (Nematoda, Spirurida) that is presently emergingin several countries. This study characterised an informative region within the mitochondrial (mtDNA) gene encoding for thecytochrome c oxidase subunit 1 (cox1) of S. lupi by Polymerase Chain Reaction (PCR)-coupled sequencing. Specimens from fivedifferent countries in Europe, Asia and Africa were examined and two different sequence variants of cox1 (i.e. haplotypes) weredetermined, displaying nucleotidic variation at 6 of 689 positions. All of these positions were invariable among all the parasiteindividuals from Europe (haplotype 1) and among the African and Asian individuals (haplotype 2), but differed between Europe andAsia/Africa. The S. lupi cox1 sequences were consistent with those of other common Spirurida previously reported at bothnucleotidic and phylogenetic levels. This study provides molecular information essential for identification of the nematode,irrespective of its life cycle stage. Crucial implications for the specific molecular diagnosis of clinical spirocercosis andinvestigation of the evolution, population genetics, ecology and epidemiology of S. lupi are discussed.[...]I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
