The generation of reactive oxygen metabolites is an important cytotoxic mechanism associated with the phagocytosis. In this study we investigated, by luminol-augmented chemiluminescence (CL), the production of reactive oxygen species (ROS) by the haemocytes of the mussel Mytilus galloprovincialis after the phagocytosis of different concentratrions of zymosan and bacteria (Escherichia coli and Vibrio alginolyticus). In order to test the specificity of the reaction, superoxide dismutase (SOD) was added to the zymosan stimulated haemocytes.The results indicate that the zymosan and bacteria phagocytosis stimulates the production of ROS; furthermore the ROS are easily quantified by luminol-enhanced chemiluminescence.The in vitro stimulation of cells with zymosan yields the highest response, whereas Vibrio alginolyticus induces the CL emission more than Escherichia coli. Forthermore increasing doses of zymosan and bacteria produce a concentration-dependent response.Finally, the inibition of the chemiluminescence response by the SOD addition confirms the specificity of the reaction.[...]
Valutazione mediante chemiluminescenza dell'attività fagocitaria degli emociti di Mitylus galloprovincialis (Lmk, 1819)
TISCAR, Pietro Giorgio;MOSCA, Francesco;MARSILIO, Fulvio
2003-01-01
Abstract
The generation of reactive oxygen metabolites is an important cytotoxic mechanism associated with the phagocytosis. In this study we investigated, by luminol-augmented chemiluminescence (CL), the production of reactive oxygen species (ROS) by the haemocytes of the mussel Mytilus galloprovincialis after the phagocytosis of different concentratrions of zymosan and bacteria (Escherichia coli and Vibrio alginolyticus). In order to test the specificity of the reaction, superoxide dismutase (SOD) was added to the zymosan stimulated haemocytes.The results indicate that the zymosan and bacteria phagocytosis stimulates the production of ROS; furthermore the ROS are easily quantified by luminol-enhanced chemiluminescence.The in vitro stimulation of cells with zymosan yields the highest response, whereas Vibrio alginolyticus induces the CL emission more than Escherichia coli. Forthermore increasing doses of zymosan and bacteria produce a concentration-dependent response.Finally, the inibition of the chemiluminescence response by the SOD addition confirms the specificity of the reaction.[...]I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
