The following contains work carried out into the preparation of an amperometric enzymatic biosensor (M.P. Byfield, R.A. Abuknesha, Biochemical aspects of biosensors. Biosens. Bioelectron. 9 (1994) 373-400; C.R. Lowe, An introduction to the concepts and technology of biosensors. Biosensors 1 (1985) 3-16; J.S. Schultz, Biosensors. Sci. Am. 8 1991 48-55) using the enzyme, lactoperoxidase (LPOD), for the detection of iodide. In this paper, a study of two major avenues which were undertaken to achieve an iodide biosensor, using electrochemical transduction techniques, are discussed. The first of these focuses on the use of LPOD in solution using both DPBS and acetate buffers, while later work has concentrated on investigations into the immobilised enzyme. The former has been shown to measure iodide concentrations of less than 0.1 mu mol/l while the latter can be used to detect iodide levels lower than 10 mu mol/l. The difficulties and drawbacks associated with both these systems are discussed. (C) 1998 Elsevier Science S.A. All rights reserved.[...]
A lactoperoxidase-based flow injection amperometric biosensor for iodide detection
COMPAGNONE, DARIO;
1998-01-01
Abstract
The following contains work carried out into the preparation of an amperometric enzymatic biosensor (M.P. Byfield, R.A. Abuknesha, Biochemical aspects of biosensors. Biosens. Bioelectron. 9 (1994) 373-400; C.R. Lowe, An introduction to the concepts and technology of biosensors. Biosensors 1 (1985) 3-16; J.S. Schultz, Biosensors. Sci. Am. 8 1991 48-55) using the enzyme, lactoperoxidase (LPOD), for the detection of iodide. In this paper, a study of two major avenues which were undertaken to achieve an iodide biosensor, using electrochemical transduction techniques, are discussed. The first of these focuses on the use of LPOD in solution using both DPBS and acetate buffers, while later work has concentrated on investigations into the immobilised enzyme. The former has been shown to measure iodide concentrations of less than 0.1 mu mol/l while the latter can be used to detect iodide levels lower than 10 mu mol/l. The difficulties and drawbacks associated with both these systems are discussed. (C) 1998 Elsevier Science S.A. All rights reserved.[...]I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
