This presentation will focus on an innovative CO -laser plotter-based strategy to synthesize metallic nanozymes as sensing elements for paper-based analytical devices (PAD). The approach consists in the single-stroke formation and anchoring of metal nanostructures, named Laser-Induced Metal nanoparticles (LIMs), with the desired design directly on cellulosic substrates. LIMs possess useful features for (bio)sensing because optically active and catalytic, and were integrated into PADs via smart benchtop technologies. As a 'kick-off' use of LIMs, a disposable LIM-PAD for the rapid and selective smartphonebased colorimetric determination of ascorbic acid (AA) will be presented. The PAD is equipped with a laser-induced platinum nanostructured catalytic paper (LIM-Pt) coupled to 3,3’,5,5’-tetramethylbenzidine (TMB) loaded fiberglass, assembled in an array format to allow the simultaneous determination of 5 samples in 1 minute. The LIM-Pt enables instantaneous TMB oxidation, given the proven oxidase-mimicking activity, ensuring the instantaneous conversion of uncolored-TMB to blue-colored TMBox, without additional reagents. AA induces a TMB-catalytic conversion inhibition resulting in a colorimetric signal ‘switch-off’. The linear AA dose-response ranged from 31 to 250 mg Kg (R²=0.992), returning a detection limit of 6 mg Kg ; analytical performance remained constant over 6 weeks (RSD=4%). The Flip-PAD exploitability was proved through AA determination in food and pharmaceutical samples, returning accurate (Rec. 92−114%; R.E. −11/+4%) and reproducible data (RSD≤10%; n=3). In case of worthy of note outcomes, other PADs based on LIM composed of different nano metals/metal-oxides will per presented. Summing up, the herein proposed laser writing strategy turns out an innovative and sustainable nanopatterning technique, prone to generate optical sensing zones useful to develop (bio)sensing strategies and manufacture within everyone's reach PADs.
Laser-induced metal nanoparticles with nanozymatic activity for paper-based analytical devices
Scroccarello A.;Della Pelle;Di Battista;El Fadil;D. Compagnone
2025-01-01
Abstract
This presentation will focus on an innovative CO -laser plotter-based strategy to synthesize metallic nanozymes as sensing elements for paper-based analytical devices (PAD). The approach consists in the single-stroke formation and anchoring of metal nanostructures, named Laser-Induced Metal nanoparticles (LIMs), with the desired design directly on cellulosic substrates. LIMs possess useful features for (bio)sensing because optically active and catalytic, and were integrated into PADs via smart benchtop technologies. As a 'kick-off' use of LIMs, a disposable LIM-PAD for the rapid and selective smartphonebased colorimetric determination of ascorbic acid (AA) will be presented. The PAD is equipped with a laser-induced platinum nanostructured catalytic paper (LIM-Pt) coupled to 3,3’,5,5’-tetramethylbenzidine (TMB) loaded fiberglass, assembled in an array format to allow the simultaneous determination of 5 samples in 1 minute. The LIM-Pt enables instantaneous TMB oxidation, given the proven oxidase-mimicking activity, ensuring the instantaneous conversion of uncolored-TMB to blue-colored TMBox, without additional reagents. AA induces a TMB-catalytic conversion inhibition resulting in a colorimetric signal ‘switch-off’. The linear AA dose-response ranged from 31 to 250 mg Kg (R²=0.992), returning a detection limit of 6 mg Kg ; analytical performance remained constant over 6 weeks (RSD=4%). The Flip-PAD exploitability was proved through AA determination in food and pharmaceutical samples, returning accurate (Rec. 92−114%; R.E. −11/+4%) and reproducible data (RSD≤10%; n=3). In case of worthy of note outcomes, other PADs based on LIM composed of different nano metals/metal-oxides will per presented. Summing up, the herein proposed laser writing strategy turns out an innovative and sustainable nanopatterning technique, prone to generate optical sensing zones useful to develop (bio)sensing strategies and manufacture within everyone's reach PADs.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
