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EnglishFourteen blood samples collected from clogs that were seropositive for Ehrlichia canis were examined For the presence of the citrate synthase gene using a highly specific and sensitive novel polymerase chain reaction assay. The assay detected E canis DNA in 3 dogs. The complete nucleotide sequence of the citrate synthase gene was determined in 2 of the test-positive samples, and represents the first sequence of the gene to be derived from Italian isolates. The sequence data displayed high identity (99.2%) between the geographically separated Italian samples and the Oklahoma strain of E canis. The high-sequence conservation revealed by molecular analysis confirmed the usefulness of the citrate synthase gene as a target for detection of E canis[...]
| Titolo: | Direct identification of Ehrlichia canis by a polymerase chain reaction method and molecular analysis of the citrate sinthase gene from various italian strains |
| Autori: | |
| Data di pubblicazione: | 2006 |
| Rivista: | |
| Abstract: | Fourteen blood samples collected from clogs that were seropositive for Ehrlichia canis were examined For the presence of the citrate synthase gene using a highly specific and sensitive novel polymerase chain reaction assay. The assay detected E canis DNA in 3 dogs. The complete nucleotide sequence of the citrate synthase gene was determined in 2 of the test-positive samples, and represents the first sequence of the gene to be derived from Italian isolates. The sequence data displayed high identity (99.2%) between the geographically separated Italian samples and the Oklahoma strain of E canis. The high-sequence conservation revealed by molecular analysis confirmed the usefulness of the citrate synthase gene as a target for detection of E canis[...] |
| Handle: | http://hdl.handle.net/11575/16721 |
| Appare nelle tipologie: | 1.1 Articolo in rivista |
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