Despite widespread use of the vaccination against Feline calicivirus (FCV) infection, FCV still represents a major cause of feline respiratory tract disease. Therefore, a new vaccination strategy is required. In this study, virus-like particles (VLPs) were produced in insect cells infected with a recombinant baculovirus containing the capsid gene of FCV The FCV VLPs were morphologically similar to the native virus and contained FCV capsid protein (VP1) with a molecular weight of approximately 60 kDa that reacted with a rabbit hyperimmune sera to FCV in Western blotting and IFA test. FCV recombinant protein expressed in the baculovirus system may be used as immunogen against feline calicivirosis could be utilized as immunogen for the recombinant vaccine[...]
Espressione della proteina del capside (VP1) del calicivirus felino mediante il sistema del baculovirus
DI MARTINO, Barbara;MARSILIO, Fulvio
2007-01-01
Abstract
Despite widespread use of the vaccination against Feline calicivirus (FCV) infection, FCV still represents a major cause of feline respiratory tract disease. Therefore, a new vaccination strategy is required. In this study, virus-like particles (VLPs) were produced in insect cells infected with a recombinant baculovirus containing the capsid gene of FCV The FCV VLPs were morphologically similar to the native virus and contained FCV capsid protein (VP1) with a molecular weight of approximately 60 kDa that reacted with a rabbit hyperimmune sera to FCV in Western blotting and IFA test. FCV recombinant protein expressed in the baculovirus system may be used as immunogen against feline calicivirosis could be utilized as immunogen for the recombinant vaccine[...]I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
