Modifications in large-scale chromatin and DNA remodeling occur during specific phases of oocyte growth to organize the genome to attend embryo/fetal development. Thus, this research was designed to study large-scale chromatin configuration, global DNA methylation, and the distribution of the major enzyme involved in DNA methylation, as DNMT1, in sheep oocytes during folliculogenesis. Moreover, a comparative study was performed between in vitro grown (IVG) oocytes and oocyte freshly collected in order to evaluate their global DNA methylation, DNMT1 distribution and chromatin organization. The results show that genomic methylation is established during the transition from preantral to early antral follicles, and that global DNA methylation is similar both in in vivo and IVG oocytes. Even if the majority of the IVG oocytes (61%) showed an immature chromatin configuration. [...]

Nuclear remodelling of in vitro grown sheep oocytes

RUSSO, Valentina;MARTELLI, Alessandra;MAURO, ANNUNZIATA;MATTIOLI, Mauro;TURRIANI, Maura;DI GIACINTO, Oriana;BERARDINELLI, Paolo;BARBONI, Barbara
2008-01-01

Abstract

Modifications in large-scale chromatin and DNA remodeling occur during specific phases of oocyte growth to organize the genome to attend embryo/fetal development. Thus, this research was designed to study large-scale chromatin configuration, global DNA methylation, and the distribution of the major enzyme involved in DNA methylation, as DNMT1, in sheep oocytes during folliculogenesis. Moreover, a comparative study was performed between in vitro grown (IVG) oocytes and oocyte freshly collected in order to evaluate their global DNA methylation, DNMT1 distribution and chromatin organization. The results show that genomic methylation is established during the transition from preantral to early antral follicles, and that global DNA methylation is similar both in in vivo and IVG oocytes. Even if the majority of the IVG oocytes (61%) showed an immature chromatin configuration. [...]
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11575/16631
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