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EnglishWe studied membrane currents in granulosa cells (GC), immediately after collection or after variable culture time in the everted-follicle wall or in the monolayer. GC in both systems express an inward calcium current (I(Ca)) with T-type kinetics and voltage dependence. GC in the everted-follicle culture express an outward potassium current (I(K)) kinetics, which remains unchanged during three days in culture. I(K) has delayed-rectifier kinetics, but is insensitive to TEA, 4-AP and apamine. GC in monolayer culture develop a new, inactivating delayed-rectifier potassium current (I(nK)), which progressively dominates as cells advance from day one to day three in culture. A similar I(nK) was recorded in large luteal cells. A possible link between luteinization and the appearance of I(nK) is hypothesized.[...]
| Titolo: | CALCIUM AND POTASSIUM CURRENTS IN PORCINE GRANULOSA CELLS MAINTAINED IN FOLLICULAR OR MONOLAYER TISSUE CULTURE. | |
| Autori: | ||
| Data di pubblicazione: | 1993 | |
| Rivista: | ||
| Abstract: | We studied membrane currents in granulosa cells (GC), immediately after collection or after variable culture time in the everted-follicle wall or in the monolayer. GC in both systems express an inward calcium current (I(Ca)) with T-type kinetics and voltage dependence. GC in the everted-follicle culture express an outward potassium current (I(K)) kinetics, which remains unchanged during three days in culture. I(K) has delayed-rectifier kinetics, but is insensitive to TEA, 4-AP and apamine. GC in monolayer culture develop a new, inactivating delayed-rectifier potassium current (I(nK)), which progressively dominates as cells advance from day one to day three in culture. A similar I(nK) was recorded in large luteal cells. A possible link between luteinization and the appearance of I(nK) is hypothesized.[...] | |
| Handle: | http://hdl.handle.net/11575/16478 | |
| Appare nelle tipologie: | 1.1 Articolo in rivista |
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