The aim of this research was to compare the efficiency in term of miniaturization, multiplexing, reproducibility and sensitivity of colorimetric microarray versus an enzyme linked oligonucleotide assay (ELONA). A competition assay was performed to explore the complex formation between 5 '-biotin NOX-B11, a special aptamer called spiegelmer, and ghrelin, a growth hormone releasing acylated peptide from stomach. The aptamer technology is a very powerful method suitable for large-scale synthesis and overcoming the disadvantages of antibodies (unstable, expensive to produce, and so on) while remaining as efficient as antibodies in binding to the target. The aptamer used in this work was based on spiegelmer oligonucleotides approach that has been considered as one of the simplest, most straightforward, and cost-effective method to develop artificial receptors.[...]
Colorimetric microarray detection system for Ghrelin Using Aptamer-Technology
MASCINI, Marcello;COMPAGNONE, DARIO
2007-01-01
Abstract
The aim of this research was to compare the efficiency in term of miniaturization, multiplexing, reproducibility and sensitivity of colorimetric microarray versus an enzyme linked oligonucleotide assay (ELONA). A competition assay was performed to explore the complex formation between 5 '-biotin NOX-B11, a special aptamer called spiegelmer, and ghrelin, a growth hormone releasing acylated peptide from stomach. The aptamer technology is a very powerful method suitable for large-scale synthesis and overcoming the disadvantages of antibodies (unstable, expensive to produce, and so on) while remaining as efficient as antibodies in binding to the target. The aptamer used in this work was based on spiegelmer oligonucleotides approach that has been considered as one of the simplest, most straightforward, and cost-effective method to develop artificial receptors.[...]I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
