TrAQ: a free behavioural tracking software for subterranean fauna

We introduce TrAQ, an innovative MATLAB-based software designed for two-dimensional tracking of unmarked single animals in Open Field video analysis, requiring minimal user intervention. TrAQ efficiently identifies the animal within a user-defined arena, offering precise quantitative measurements of the body centroid and the positions of two extremities. Users can select any arbitrary arena shape, and the software automatically calculates a comprehensive range of kinematic behavioral parameters. An optional shape erosion feature enhances its flexibility in removing animal’s appendices or small debris. Developed by the University of L'Aquila, TrAQ is a free, versatile application that is not species-specific and can be adapted to various 2D behavioral assessments with no need for dedicated video hardware. Initially applied to groundwater copepods and later tested on mammals, it is suitable for both aquatic and terrestrial animals of any size and colour. The quantitative results have been successfully validated against commercial software. TrAQ is particularly useful for describing the locomotion traits of subterranean fauna, providing valuable insights into their movement patterns. To ensure userfriendliness, we have included intuitive GUIs and a detailed instruction manual outlining the initial steps. During our presentation, we will demonstrate the software's basic functionality and provide a download link. The TrAQ script can be customized to meet specific users’ requirements by contacting the developers at davide.dicenso89@gmail.com and angelo.galante@univaq.it.