Proteomics Mass Spectrometry Methods: Chapter 8 - Sample preparation for proteomics and mass spectrometry from animal samples

Proteomics, although being actively developed and applied in a diverse range of areas including human biomedicine or plant science, has been limited in animal science. Gathering information from studies conducted on farm animal species will not only benefit the field of animal science but it will help in the understanding of the complex biological processes involved in human health and disease. Here we provide a simple and efficient protocol for proteins extraction from muscle tissue (exudate collected postmortem following centrifugation). Moreover, in this chapter we describe a Filter Aided Sample Preparation (FASP) protocol optimized for animal biological material. This method uses standard filtration device with different pore size to retain proteins with bigger size, while detergents and impurities pass through. The FASP method has been successfully employed in animal specimens for proteins purification and digestion expanding the breadth of achievable proteome coverage in animal science.