Recently the transient receptor potential vanilloid type 1 (TRPV1) has been described to be involved inthe capacitation, the process leading mammalian spermatozoa to acquire full fertilizing ability withinthe female genital tract. TRPV1 immunolocalization during capacitation and the effect of TRPV1 inhibitionby the capsazepin (CPZ) or activation by the capsaicin (CPS) on membrane resting potential, calciumclearance and actin polymerization have been investigated. It was found that the capacitation promotedthe translocation of TRPV1 from the post-acrosomal to the apical region of sperm head. Moreover the CPZinduced the progressive drop in intracellular Ca2+ levels during capacitation and the inhibition of actinpolymerization in the acrosomal region. On the contrary, the CPS caused the sperm membrane depolarizationdue to the Na+ influx and the consequent voltage gated calcium channels (VGCC) opening. Inconclusion it was suggested that TRPV1 channels modulate the major pathways involved in capacitation.[...]
Role of TRPV1 channels in boar spermatozoa acquisition of fertilizing ability
BERNABO', NICOLA;MATTIOLI, Mauro;BARBONI, Barbara
2010-01-01
Abstract
Recently the transient receptor potential vanilloid type 1 (TRPV1) has been described to be involved inthe capacitation, the process leading mammalian spermatozoa to acquire full fertilizing ability withinthe female genital tract. TRPV1 immunolocalization during capacitation and the effect of TRPV1 inhibitionby the capsazepin (CPZ) or activation by the capsaicin (CPS) on membrane resting potential, calciumclearance and actin polymerization have been investigated. It was found that the capacitation promotedthe translocation of TRPV1 from the post-acrosomal to the apical region of sperm head. Moreover the CPZinduced the progressive drop in intracellular Ca2+ levels during capacitation and the inhibition of actinpolymerization in the acrosomal region. On the contrary, the CPS caused the sperm membrane depolarizationdue to the Na+ influx and the consequent voltage gated calcium channels (VGCC) opening. Inconclusion it was suggested that TRPV1 channels modulate the major pathways involved in capacitation.[...]I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
