Listeriosis is a zoonosis caused by Listeria monocytogenes, which is a foodborne pathogen posing a significant health risk, especially to individuals with weakened immune systems, including pregnant women, newborns, young children, and the elderly. The consumption of several food products has been linked to numerous outbreaks, including raw milk, soft cheeses, processed meats, seafood, and ready-to-eat foods like salads and raw vegetables. One of the concerning aspects of L. monocytogenes is its ability to thrive and reproduce in a wide range of environmental conditions, which poses challenges for food safety and contamination control. This adaptability allows the pathogen to persist in conditions with low pH, refrigeration temperatures, and high salt concentrations [1]. The following study aims to identify differentially expressed genes and pathogenic proteins encoded by L. monocytogenes during its exposure to stress growth conditions (37°C, 0.5%, pH 7 vs A2 - 12°C, 7.0%, pH 5.5). Bacterial cells were collected during late exponential growth phase to extract, purify and quantify RNA and protein samples as well. Transcripts were analyzed by RNAseq technique while proteins by nLC-MS/MS approach. Proteins identification required a minimum of 2 peptides per protein against the L. monocytogenes Uniprot database. Differential expression results were obtained by TopHat and Cufflinks software and were correlated with proteomic data, which were analyzed and clustered using an immunoinformatic pipeline and STRING v11.05, respectively. By the analysis performed it was identified a specific panel of genes which encoded for several specific proteins associated with cold tolerance (lmo0823), acidic (lmo2005) and oxidative stress. Furthermore all the proteins identified are involved in pathogenic and virulent pathways and most of them are well known to be regulated by Prfa gene (lmo2157). These results are supported by prior literature reports and could be useful for future research focused on understanding the pathogenesis of listeriosis.

Study of Listeria monocytogenes: a proteomic and transcriptomic approach

Schirone M.;
2023-01-01

Abstract

Listeriosis is a zoonosis caused by Listeria monocytogenes, which is a foodborne pathogen posing a significant health risk, especially to individuals with weakened immune systems, including pregnant women, newborns, young children, and the elderly. The consumption of several food products has been linked to numerous outbreaks, including raw milk, soft cheeses, processed meats, seafood, and ready-to-eat foods like salads and raw vegetables. One of the concerning aspects of L. monocytogenes is its ability to thrive and reproduce in a wide range of environmental conditions, which poses challenges for food safety and contamination control. This adaptability allows the pathogen to persist in conditions with low pH, refrigeration temperatures, and high salt concentrations [1]. The following study aims to identify differentially expressed genes and pathogenic proteins encoded by L. monocytogenes during its exposure to stress growth conditions (37°C, 0.5%, pH 7 vs A2 - 12°C, 7.0%, pH 5.5). Bacterial cells were collected during late exponential growth phase to extract, purify and quantify RNA and protein samples as well. Transcripts were analyzed by RNAseq technique while proteins by nLC-MS/MS approach. Proteins identification required a minimum of 2 peptides per protein against the L. monocytogenes Uniprot database. Differential expression results were obtained by TopHat and Cufflinks software and were correlated with proteomic data, which were analyzed and clustered using an immunoinformatic pipeline and STRING v11.05, respectively. By the analysis performed it was identified a specific panel of genes which encoded for several specific proteins associated with cold tolerance (lmo0823), acidic (lmo2005) and oxidative stress. Furthermore all the proteins identified are involved in pathogenic and virulent pathways and most of them are well known to be regulated by Prfa gene (lmo2157). These results are supported by prior literature reports and could be useful for future research focused on understanding the pathogenesis of listeriosis.
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11575/140440
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