Parameters of cellular and humoral immunity wereanalysed in Dicentrarchus labrax to evaluate themodulatory activity of Ergosan®, an algal extractcontaining 1% alginic acid. Four cycles using normal fishfeed formulation supplemented with 0.5% Ergosan wereperformed at 60-days intervals (15-days treatment + 45-days suspension). Serum complement (C), lysozyme andtotal proteins and tissue HSP were measured 15, 30 and45 days after the first 15-days feeding cycle (short-term)and 45 days after each feeding cycle over a 35-weeksperiod (long-term). The percentages of B and Tlymphocytes in PBL and gut were measured over longtermtrial using mAbs against homologous Ig and T cells.Short-term alginic acid treatment raised serum C activity(p<0.05) at 15 days and serum C and lysozyme, gill andliver HSP (p<0.05) at 30 days. No significant differenceswith control group were found at 45 days. Over the longtermperiod, innate and specific immune parameters,survival, growth performances and conversion index didnot differ in treated and control fish.An experimental group received an oral Vibrioanguillarum vaccine (AVL, UK) according to the followingadministration protocol: 5-days vaccine and 0.5%Ergosan diet, 5-days normal diet, 5-days vaccine andErgosan diet (vaccination cycle, total dose: 0.2 ml/fish).Controls received the same dose of vaccine and were fedwith normal diet throughout the cycle. Total and specificserum Ig, C activity and percentages of lymphocytes inPBL and gut were measured 30 days after the start of thevaccination cycle.Oral vaccine raised significantly PBL Ig+ cells (p<0.05)and gut T-cells (p<0.01) in the Ergosan group. The titre ofspecific serum anti-Vibrio Ig was higher (p<0.05) inErgosan group compared with controls, while total Ig wereunmodified. These effects were accompanied by a rise ofPBL (T-cells +12%, B-cells +27%), intestine lymphocytes(T-cells +17%) and serum C (+19%) compared withvaccinated controls.The present studies in the sea bass indicate veryinteresting immunostimulatory properties of alginate.This work was granted by MIPAF Projects 5C68 and5C116.[...]
Immunity in Dicentrarchus labrax culture: in vivo effects of arginate immunostimulation.
TISCAR, Pietro Giorgio;
2005-01-01
Abstract
Parameters of cellular and humoral immunity wereanalysed in Dicentrarchus labrax to evaluate themodulatory activity of Ergosan®, an algal extractcontaining 1% alginic acid. Four cycles using normal fishfeed formulation supplemented with 0.5% Ergosan wereperformed at 60-days intervals (15-days treatment + 45-days suspension). Serum complement (C), lysozyme andtotal proteins and tissue HSP were measured 15, 30 and45 days after the first 15-days feeding cycle (short-term)and 45 days after each feeding cycle over a 35-weeksperiod (long-term). The percentages of B and Tlymphocytes in PBL and gut were measured over longtermtrial using mAbs against homologous Ig and T cells.Short-term alginic acid treatment raised serum C activity(p<0.05) at 15 days and serum C and lysozyme, gill andliver HSP (p<0.05) at 30 days. No significant differenceswith control group were found at 45 days. Over the longtermperiod, innate and specific immune parameters,survival, growth performances and conversion index didnot differ in treated and control fish.An experimental group received an oral Vibrioanguillarum vaccine (AVL, UK) according to the followingadministration protocol: 5-days vaccine and 0.5%Ergosan diet, 5-days normal diet, 5-days vaccine andErgosan diet (vaccination cycle, total dose: 0.2 ml/fish).Controls received the same dose of vaccine and were fedwith normal diet throughout the cycle. Total and specificserum Ig, C activity and percentages of lymphocytes inPBL and gut were measured 30 days after the start of thevaccination cycle.Oral vaccine raised significantly PBL Ig+ cells (p<0.05)and gut T-cells (p<0.01) in the Ergosan group. The titre ofspecific serum anti-Vibrio Ig was higher (p<0.05) inErgosan group compared with controls, while total Ig wereunmodified. These effects were accompanied by a rise ofPBL (T-cells +12%, B-cells +27%), intestine lymphocytes(T-cells +17%) and serum C (+19%) compared withvaccinated controls.The present studies in the sea bass indicate veryinteresting immunostimulatory properties of alginate.This work was granted by MIPAF Projects 5C68 and5C116.[...]I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
