Several studies point out on effects of indoor pollutants (silica particles, Pb, Ni, Zn, Si, ozone, carbon monoxide and nitrogen oxides). The work aim is a multidisciplinary study of silica ultrafine particles (<2.5μ) in the presence or not of ozone O3 and their potential cyto-genotoxicity evaluated by MTS, Micronucleus and Comet test in two cell lines. We analyzed A549 (adenocarcinoma human alveolar basal epithelial cells) and HS 27(normal human fibroblasts) exposed under dynamic conditions by a simulator IRC under stream of ozone (120 ppb) and silica particles (40μg/h). A549 cells viability at 48 hours and 72 hours exposition to silica alone or silica plus ozone doesn’t differ in respect to the control. The viability trend in the Hs27 cells did not show any significant changes in treated samples compared to the control in all conditions. The micronucleus and the comet tests, showed in both the cell types exposed to silica alone or plus ozone, a significant increase in the number of micronuclei and in the tail DNA % in respect to the control even if at different times/cell type. We found that silica alone or plus O3 provokes genotoxic effects more evident in A549 cancer cells than in normal fibroblasts Hs27 ones. Final output will be to obtain a picture of the role of silica particles/ozone in the indoor air quality taking account of the potential simultaneous co-toxicant action.
Silica Nanoparticles and Ozone: an evaluation of in vitro cytotoxicity and genotoxicity in a model of indoor air exposition
Aloisi M.;
2021-01-01
Abstract
Several studies point out on effects of indoor pollutants (silica particles, Pb, Ni, Zn, Si, ozone, carbon monoxide and nitrogen oxides). The work aim is a multidisciplinary study of silica ultrafine particles (<2.5μ) in the presence or not of ozone O3 and their potential cyto-genotoxicity evaluated by MTS, Micronucleus and Comet test in two cell lines. We analyzed A549 (adenocarcinoma human alveolar basal epithelial cells) and HS 27(normal human fibroblasts) exposed under dynamic conditions by a simulator IRC under stream of ozone (120 ppb) and silica particles (40μg/h). A549 cells viability at 48 hours and 72 hours exposition to silica alone or silica plus ozone doesn’t differ in respect to the control. The viability trend in the Hs27 cells did not show any significant changes in treated samples compared to the control in all conditions. The micronucleus and the comet tests, showed in both the cell types exposed to silica alone or plus ozone, a significant increase in the number of micronuclei and in the tail DNA % in respect to the control even if at different times/cell type. We found that silica alone or plus O3 provokes genotoxic effects more evident in A549 cancer cells than in normal fibroblasts Hs27 ones. Final output will be to obtain a picture of the role of silica particles/ozone in the indoor air quality taking account of the potential simultaneous co-toxicant action.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
