Horseradish peroxidase and the substrate 3,3',5,5'-tetramethylbenzidine have been incorporated into a solid carbon paste electrode for the development of a H2O2 probe. The electrode exhibited a detection limit of 10(-8) mol/L H2O2 and a linearity extended over three orders of magnitude. Glucose oxidase was then used in the paste or covalently immobilized onto a preactivated membrane for the detection of glucose. Using both immobilization procedures the measurement of glucose in the micromolar range and an extended linearity was achieved.[...]
Sub-micromolar detection of hydrogen peroxide at a peroxidase/tetramethylbenzidine solid carbon paste electrode
COMPAGNONE, DARIO;
1998-01-01
Abstract
Horseradish peroxidase and the substrate 3,3',5,5'-tetramethylbenzidine have been incorporated into a solid carbon paste electrode for the development of a H2O2 probe. The electrode exhibited a detection limit of 10(-8) mol/L H2O2 and a linearity extended over three orders of magnitude. Glucose oxidase was then used in the paste or covalently immobilized onto a preactivated membrane for the detection of glucose. Using both immobilization procedures the measurement of glucose in the micromolar range and an extended linearity was achieved.[...]File in questo prodotto:
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