Martina Franca donkey is an Italian native breed originally from the Puglia region in South Italy. These donkeys are mostly raised in groups that live outdoors throughout the year. The “Cuboni Reaction” is a test based on a chemical reaction that detects free estrogen in mares’ urine using hydrochloric acid, benzene and sulfuric acid; it allows for a late pregnancy diagnosis. To the authors’ knowledge, there is only one paper in literature reproducing this technique on asinine species. The purpose of this study is to apply the Cuboni Reaction test to the Martina Franca donkey to confirm the effectiveness of this method in asinine species as well. 18 Martina Franca jennies, whose pregnancy has been confirmed through transrectal palpations and ultrasound examinations, were subjected to urine collection to confirm the pregnancy diagnosis by the Cuboni Reaction. Data collection were taken at G 120, G 135 and G 150, to identify the moment when the reaction becomes positive. Other samples were taken from G 270, every 15 days until a negative result was obtained. Furthermore, the test was carried out on 2 non-pregnant jennies in order to assess reaction specificity. Two collection techniques were used: collection from spontaneous urination was performed in jennies not accustomed to the medical procedures; and collection from bladder catheterization in animals accustomed to the medical procedures. This work shows that Cuboni Reaction is over 50% sensitive from 120th day of pregnancy and 100% sensitive from 150th to 300th day. However, the sensitivity of Cuboni Reaction is less than 40% over 315th day of pregnancy; it also loses specificity detecting false negatives from day 315. Cuboni Reaction, despite having been overtaken by new technologies for pregnancy diagnosis, still proves useful, particularly in Martina Franca donkey farms, where animals are raised in fields and are not used to handling. This method can still be used as a late pregnancy test in jennies for which the ovulation day is not known but in which there is a supposed date of mating; it is safe for the involved animals, easy to carry out by the breeder and provides results in a short time.
Cuboni reaction: Non-invasive late pregnancy test in martina franca jennies
Bucci R.;Giangaspero B. A.;Robbe D.;Ponzio P.;Manetta A. C.;Di Giuseppe L.;
2020-01-01
Abstract
Martina Franca donkey is an Italian native breed originally from the Puglia region in South Italy. These donkeys are mostly raised in groups that live outdoors throughout the year. The “Cuboni Reaction” is a test based on a chemical reaction that detects free estrogen in mares’ urine using hydrochloric acid, benzene and sulfuric acid; it allows for a late pregnancy diagnosis. To the authors’ knowledge, there is only one paper in literature reproducing this technique on asinine species. The purpose of this study is to apply the Cuboni Reaction test to the Martina Franca donkey to confirm the effectiveness of this method in asinine species as well. 18 Martina Franca jennies, whose pregnancy has been confirmed through transrectal palpations and ultrasound examinations, were subjected to urine collection to confirm the pregnancy diagnosis by the Cuboni Reaction. Data collection were taken at G 120, G 135 and G 150, to identify the moment when the reaction becomes positive. Other samples were taken from G 270, every 15 days until a negative result was obtained. Furthermore, the test was carried out on 2 non-pregnant jennies in order to assess reaction specificity. Two collection techniques were used: collection from spontaneous urination was performed in jennies not accustomed to the medical procedures; and collection from bladder catheterization in animals accustomed to the medical procedures. This work shows that Cuboni Reaction is over 50% sensitive from 120th day of pregnancy and 100% sensitive from 150th to 300th day. However, the sensitivity of Cuboni Reaction is less than 40% over 315th day of pregnancy; it also loses specificity detecting false negatives from day 315. Cuboni Reaction, despite having been overtaken by new technologies for pregnancy diagnosis, still proves useful, particularly in Martina Franca donkey farms, where animals are raised in fields and are not used to handling. This method can still be used as a late pregnancy test in jennies for which the ovulation day is not known but in which there is a supposed date of mating; it is safe for the involved animals, easy to carry out by the breeder and provides results in a short time.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
