Development of hybrid nanocarrier library based biomaterials for cancer therapy

Introduction: The major challenges are requested to improve the properties of nanoparticles injected systemically: i) the increase of payloads inside nanoparticles, ii) the controlled release of payloads, iii) the decrease of interaction and uptake with mononuclear phagocyte system cells, iv) the selective targeting. Geometry and size of the therapeutic nanoparticles play a crucial role for their adhesion to tumor vasculature1. Discoidal non-spherical nanoparticles interact better with the fenestrated endothelium of tumor tissue with respect to spherical nanoparticles. Recently, discoidal nanoparticles have been developed to delivery payloads for anticancer therapy as well as cardiovascular diseases2. The aim of this project is of the synthesis of discoidal and hybrid nanocarrier (DHN) obtained by combining biomaterials, e.g. lipid/polymer, and extracellular vesicles for the treatment of Hepatoma and related-diseases. In particular, DHN shall allow to cure the liver diseases (i.e., cancer) and to stimulate the regeneration of tissue. Materials and Methods: DHN will be designed, synthesized and physicochemical characterize during different techniques such as DLS, NTA,TEM, FT-IR, LC ms/ms, and HPLC. In vitro and in vivo experiment will be carried out to test the toxicity and pre-clinic efficacy in suitable hepatic cellular lines and murine models. Conclusion: There resulting DHN may represent a new nanomedicine for the treatment of Hepatoma and related-diseases.