Molecular-based approaches are rapidly developing in medicine for the evaluation of physiological and pathological conditions and discovery of new biomarkers in prevention and therapy. Fatty acid diversity and roles in health and disease in humans are topical subjects of lipidomics. In particular, membrane fatty acid-based lipidomics provides molecular data of relevance in the study of human chronic diseases, connecting metabolic, and nutritional aspects to health conditions. In veterinarymedicine,membrane lipidomics, and fatty acid profiles have not been developed yet in nutritional approaches to health and in disease conditions. Using a protocol widely tested in human profiling, in the present study erythrocyte membrane lipidome was examined in 68 clinically healthy dogs, with different ages, sex, and sizes. In particular, a cluster composed of 10 fatty acids, present in membrane glycerophospholipids and representative of structural and functional properties of cell membrane, was chosen, and quantitatively analyzed. The interval values and distribution for each fatty acid of the cluster were determined, providing the first panel describing the healthy dog lipidomic membrane profile, with interesting correlation to bodyweight increases. This molecular information can be advantageously developed as benchmark in veterinarymedicine for the evaluation of metabolic and nutritional status in healthy and diseased dogs.
The Erythrocyte Membrane Lipidomeof Healthy Dogs: Creating a Benchmark of Fatty Acid Distribution and Interval Values
Paraskevi Prasinou;Paolo E. Crisi;Morena Di Tommaso;Alessandro Gramenzi;Benedetta Belà;Francesca De Santis;Andrea Boari
;
2020-01-01
Abstract
Molecular-based approaches are rapidly developing in medicine for the evaluation of physiological and pathological conditions and discovery of new biomarkers in prevention and therapy. Fatty acid diversity and roles in health and disease in humans are topical subjects of lipidomics. In particular, membrane fatty acid-based lipidomics provides molecular data of relevance in the study of human chronic diseases, connecting metabolic, and nutritional aspects to health conditions. In veterinarymedicine,membrane lipidomics, and fatty acid profiles have not been developed yet in nutritional approaches to health and in disease conditions. Using a protocol widely tested in human profiling, in the present study erythrocyte membrane lipidome was examined in 68 clinically healthy dogs, with different ages, sex, and sizes. In particular, a cluster composed of 10 fatty acids, present in membrane glycerophospholipids and representative of structural and functional properties of cell membrane, was chosen, and quantitatively analyzed. The interval values and distribution for each fatty acid of the cluster were determined, providing the first panel describing the healthy dog lipidomic membrane profile, with interesting correlation to bodyweight increases. This molecular information can be advantageously developed as benchmark in veterinarymedicine for the evaluation of metabolic and nutritional status in healthy and diseased dogs.File | Dimensione | Formato | |
---|---|---|---|
Supplementary Material_The Erythrocyte Membrane Lipidome of Healthy Dogs Creating a Benchmark of Fatty Acid Distribution and Interval Values.pdf
accesso aperto
Descrizione: Supplementary Material
Tipologia:
Documento in Post-print
Licenza:
PUBBLICO - Pubblico con Copyright
Dimensione
2.22 MB
Formato
Adobe PDF
|
2.22 MB | Adobe PDF | Visualizza/Apri |
Fvets-07-00502_The Erythrocyte Membrane Lipidome of Healthy Dogs Creating a Benchmark of Fatty Acid Distribution and Interval Values.pdf
accesso aperto
Descrizione: Articolo principale
Tipologia:
Documento in Post-print
Licenza:
PUBBLICO - Pubblico con Copyright
Dimensione
1.18 MB
Formato
Adobe PDF
|
1.18 MB | Adobe PDF | Visualizza/Apri |
I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.