Listeria monocytogenes is an ubiquitous bacterium responsible of food contamination causing often severe consequences in immunocompromised or pregnant people. The infection is related to the consumption of ready-to-eat food but also fish, meat, dairy and vegetables. Most important, L. monocytogenes often exhibits persistence and resistant features. In 2015 a severe outbreak occurred in central Italy causing several infected and death people. The responsible strain was identified in “headcheese” samples which, from the point of view of the bacteria, can be considered a stressor environment in terms of storage temperature, pH, salt and concentration. Using a transcriptomic approach, based on a differential expression study, we were able to identify key genes and protein modules responsible of such a fitness. Although expensive and difficult to apply routinely, RNAseq analysis revealed its usefulness in functional characterization of pathogens strains.
TRASCRITTOMICA COME FRAMEWORK NELLO STUDIO DI NUOVI BIOMARCATORI DI RESISTENZA A STRESS AMBIENTALI: L’ESPERIENZA DI LISTERIA MONOCYTOGENES
Di Lollo V.
;
2019-01-01
Abstract
Listeria monocytogenes is an ubiquitous bacterium responsible of food contamination causing often severe consequences in immunocompromised or pregnant people. The infection is related to the consumption of ready-to-eat food but also fish, meat, dairy and vegetables. Most important, L. monocytogenes often exhibits persistence and resistant features. In 2015 a severe outbreak occurred in central Italy causing several infected and death people. The responsible strain was identified in “headcheese” samples which, from the point of view of the bacteria, can be considered a stressor environment in terms of storage temperature, pH, salt and concentration. Using a transcriptomic approach, based on a differential expression study, we were able to identify key genes and protein modules responsible of such a fitness. Although expensive and difficult to apply routinely, RNAseq analysis revealed its usefulness in functional characterization of pathogens strains.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
