In vitro antibacterial efficacy of a based blackcurrant oil shampoo against: Staphylococcus pseudintermedius, Staphylococcus aureus, Escherichia coli and Pseudomonas aeruginosa

In the last years the increase of antibiotic resistance has introduced the must of new therapeutic strategies. The use of topical anti-septic for dogs' superficial pyoderma has proven equivalent efficacy compared to systemic antibiotic. The aim of this study was to test the in vitro antibacterial efficacy of a shampoo/conditioner containing blackcurrant seed oil, piroctone olamine, 18-beta-glycyrrhetinic acid and ceramides. Fifty bacterial strains, obtained by dogs with superficial pyoderma, were selected. They were represented by: 10 Staplylococcus pseudintermedius, 10 Staphylococcus aureus, 10 Staphylococcus pseudintermedius methicillin-resistant, 10 Es-cherichia coli, 10 Pseudomonas aeruginosa. A concentration of 106 UCF/mL of selected bacteria was usedfor serial dilution in a sterile plate with 96 wells; these were filled with 100 μ l of the tested shampoo (Ribes Pet Shampoo® Ultra, NBF Lines, Milan) with progressive dilution from 1:2 to 1:256. After the incubation at 37°C for 30 minutes, a rate of 10 μ lwas taken from any single well. Each one was sow on Tryptone Scya agar with 5% of sheep blood and incubated for 24 hours to evaluate the MIC. The product showed bactericidal activity till a dilu-tion of 1:16 for all strains. At 1:32 dilution, grown 4 MRSP, 4 P. aeruginosa, 2 SIG e 2 E. coli; at 1:64 dilution, 6 S1G, 6 S. aureus, 4 MRSP, P. aeruginosa and E. coli; at 1:128 dilution were present 4 E. coli, 2 MRSP, S. pseudintermedius, S. aureus e P. aeruginosa. Finally at 1:256 dilution, 2 strains of S. aureus grown.