Introduction. Aberrant placentation occurs early during embryonic development after assisted reproductive techniques. To further understand this failure, vascular morphometry of ovine placentas and embryo growth after in vitro activation (IVA) and in vitro fertilization (IVF) at 20 and 22 days of gestation was performed.Results. Crown-ramp measure (mm) after in vitro manipulation is reduced at 20 (CTR-controls: 3.84; IVF: 3.48; IVA: 3.46) and 22 days (CTR: 4.42; IVF: 3.84; IVA: 3.74), as well as placental vessel number/field (20-22 days: IVF: 1.25-1.93; IVA: 1.24-1.71; CTR: 3.11-3.48). At 20 d, stage 1 vessels (early vasculogenesis) were prevalent in IVA samples (IVA: 26.67% of total vessels; IVF: 10.55%; CTR: 3.54%), stage 2 (early tube formation) in IVF (IVF: 77.82%; CTR: 70.31%; IVA: 66.33%) and stage 3 (late vasculogenesis) in CTR (CTR: 26.15%; IVF: 11.63%; IVA: 10%). At 22 d, more vessels in stages 1 (18.18%) and 2 (72.72%) occur after IVA than IVF (1: 5.24%; 2: 64.51%) and CTR (1: 2.62%; 2: 53.59%), while CTR had more stage 3 vessels (43.79%) than IVF (30.25%) and IVA (9.1%). Discussion. In vitro manipulation leads to delayed maturation and reduced density of placental vessels, that affect post-implantation embryo growth. [...]
IMPAIRED PLACENTAL VASCULARIZATION AND EMBRYO GROWTH AFTER IN-VITRO MANIPULATION IN SHEEP: A MORPHOMETRIC STUDY
DELLA SALDA, Leonardo
2012-01-01
Abstract
Introduction. Aberrant placentation occurs early during embryonic development after assisted reproductive techniques. To further understand this failure, vascular morphometry of ovine placentas and embryo growth after in vitro activation (IVA) and in vitro fertilization (IVF) at 20 and 22 days of gestation was performed.Results. Crown-ramp measure (mm) after in vitro manipulation is reduced at 20 (CTR-controls: 3.84; IVF: 3.48; IVA: 3.46) and 22 days (CTR: 4.42; IVF: 3.84; IVA: 3.74), as well as placental vessel number/field (20-22 days: IVF: 1.25-1.93; IVA: 1.24-1.71; CTR: 3.11-3.48). At 20 d, stage 1 vessels (early vasculogenesis) were prevalent in IVA samples (IVA: 26.67% of total vessels; IVF: 10.55%; CTR: 3.54%), stage 2 (early tube formation) in IVF (IVF: 77.82%; CTR: 70.31%; IVA: 66.33%) and stage 3 (late vasculogenesis) in CTR (CTR: 26.15%; IVF: 11.63%; IVA: 10%). At 22 d, more vessels in stages 1 (18.18%) and 2 (72.72%) occur after IVA than IVF (1: 5.24%; 2: 64.51%) and CTR (1: 2.62%; 2: 53.59%), while CTR had more stage 3 vessels (43.79%) than IVF (30.25%) and IVA (9.1%). Discussion. In vitro manipulation leads to delayed maturation and reduced density of placental vessels, that affect post-implantation embryo growth. [...]I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
