Amniotic epithelial stem cells (AECs) retain great potential in cell-based regenerative medicine for their plasticity, immune tolerance and anti-inflammatory properties, thus, ideal for allo and xeno-transplantation settings.Their performance would be increased by creating AECs engineered scaffolds allowing the tissue regeneration, mechanical and structural preservation. PLGA, PCL, and PLA nonwovens electrospun scaffolds were tested for oAECs’ biocompatibility. In particular, it was studied the correlation between biopolymer characteristics and AECs’ attachment, spatial organization and proliferation. Results demonstrate that oAECs and electrospun PCL, PLA and PLGA scaffolds are biocompatible. This positive interaction can be attributed to the geometrical features, including scaffold pore size and biopolymer properties which support oAECs cell adhesion, spatial organization, survival and proliferation.In conclusion, the investigated biocompatible electrospun matrices, in particular PLGA, represent promising bioscaffolds in AECs-based regenerative medicine.
Positive interaction between the amniotic epithelial stem cells and electrospun poly(lactide-coglycolide), poly(ε-caprolactone), poly(lactic acid)
RUSSO, Valentina;DI MARCANTONIO, LISA;VALBONETTI, Luca;MAURO, ANNUNZIATA;MARTELLI, Alessandra;BARBONI, Barbara
2016-01-01
Abstract
Amniotic epithelial stem cells (AECs) retain great potential in cell-based regenerative medicine for their plasticity, immune tolerance and anti-inflammatory properties, thus, ideal for allo and xeno-transplantation settings.Their performance would be increased by creating AECs engineered scaffolds allowing the tissue regeneration, mechanical and structural preservation. PLGA, PCL, and PLA nonwovens electrospun scaffolds were tested for oAECs’ biocompatibility. In particular, it was studied the correlation between biopolymer characteristics and AECs’ attachment, spatial organization and proliferation. Results demonstrate that oAECs and electrospun PCL, PLA and PLGA scaffolds are biocompatible. This positive interaction can be attributed to the geometrical features, including scaffold pore size and biopolymer properties which support oAECs cell adhesion, spatial organization, survival and proliferation.In conclusion, the investigated biocompatible electrospun matrices, in particular PLGA, represent promising bioscaffolds in AECs-based regenerative medicine.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
