A toxicity test was realized to assess the effects of the herbicide Propanil (3-4 dicloropropionanilide) on tissues and cells of chub.Thirtysix specimens of Leuciscus cephalus (L.) were exposed to different concentrations of Propanil (0 mg/L; 3.16 mg/L; 6.31 mg/L; 12.6mg/L; 25.1 mg/L) and dissected at 0, 24 or 48 hours after exposure. Pieces of the main organs (heart, gills, liver, kidney and intestine) fromeach fish were fixed and processed for light and electron microscopy. The present study was focused on rodlet cells (RCs). The occurenceand distribution of these cells were affected by Propanil: increasing concentrations of the herbicide were associated with a linearaugmentation of RCs in the gills and with an exponential depletion of the same cells in the arteriosus bulbus. Moreover, in different organsof the exposed chub were find rodlet cells with ultrastructural changes and signs of degeneration. Finally, these results confirm that RCsare very sensitive to stress and so could be used as bioindicator of water quality.[...]
Osservazioni istologiche ed ultrastrutturali in cavedani, Leuciscus cephalus trattati con un erbicida
MANERA, Maurizio;
2004-01-01
Abstract
A toxicity test was realized to assess the effects of the herbicide Propanil (3-4 dicloropropionanilide) on tissues and cells of chub.Thirtysix specimens of Leuciscus cephalus (L.) were exposed to different concentrations of Propanil (0 mg/L; 3.16 mg/L; 6.31 mg/L; 12.6mg/L; 25.1 mg/L) and dissected at 0, 24 or 48 hours after exposure. Pieces of the main organs (heart, gills, liver, kidney and intestine) fromeach fish were fixed and processed for light and electron microscopy. The present study was focused on rodlet cells (RCs). The occurenceand distribution of these cells were affected by Propanil: increasing concentrations of the herbicide were associated with a linearaugmentation of RCs in the gills and with an exponential depletion of the same cells in the arteriosus bulbus. Moreover, in different organsof the exposed chub were find rodlet cells with ultrastructural changes and signs of degeneration. Finally, these results confirm that RCsare very sensitive to stress and so could be used as bioindicator of water quality.[...]I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
