Introduction: PDGFRα and PDGFRβ are tyrosine kinases receptors overexpressed in 70-80% of human osteosarcoma (OSA) that represent a suitable target for clinical use of specific kinases inhibitors. Canine OSA is considered a model in comparative oncology. In this study we investigated PDGFRα and PDGFRβ expression in canine OSA tissues and primaries canine OSA cell lines.Materials and Methods: PDGFRα and PDGFRβ transcripts was evaluated in 7 OSA cells line by q-PCR. PDGFRα and PDGFRβ expression was evaluated by western blot on 7 cells lines lysates and by immunohistochemistry on 28 cases of canine OSA.Results:Molecular studies revealed that PDGFRα and PDGFRβ transcripts are over-expressed respectively in 4/7 OSA cells line and in 2/7 OSA cell lines if compared to normal osteoblastic cell lines. Immunohistochemistry revealed that canine PDGFRα and PDGFRβ are expressed in 71,4% and 82,1% respectively Conclusion: These data showed that expression and distribution of the PDGFRα and PDGFRβ in canine osteosarcomas are similar to human suggesting the potential therapeutic target of this receptors and remarking the important role of canine model in testing innovative approaches for human osteosarcomas therapies. [...]
Co-expression of PDGFR Alpha and Beta in Canine Osteosarcomas Cell Lines and Tissues: New Targets for Innovative Therapeutic Strategies.
DELLA SALDA, Leonardo;
2012-01-01
Abstract
Introduction: PDGFRα and PDGFRβ are tyrosine kinases receptors overexpressed in 70-80% of human osteosarcoma (OSA) that represent a suitable target for clinical use of specific kinases inhibitors. Canine OSA is considered a model in comparative oncology. In this study we investigated PDGFRα and PDGFRβ expression in canine OSA tissues and primaries canine OSA cell lines.Materials and Methods: PDGFRα and PDGFRβ transcripts was evaluated in 7 OSA cells line by q-PCR. PDGFRα and PDGFRβ expression was evaluated by western blot on 7 cells lines lysates and by immunohistochemistry on 28 cases of canine OSA.Results:Molecular studies revealed that PDGFRα and PDGFRβ transcripts are over-expressed respectively in 4/7 OSA cells line and in 2/7 OSA cell lines if compared to normal osteoblastic cell lines. Immunohistochemistry revealed that canine PDGFRα and PDGFRβ are expressed in 71,4% and 82,1% respectively Conclusion: These data showed that expression and distribution of the PDGFRα and PDGFRβ in canine osteosarcomas are similar to human suggesting the potential therapeutic target of this receptors and remarking the important role of canine model in testing innovative approaches for human osteosarcomas therapies. [...]I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
