Amniotic membranes and amniotic fluid have attracted increased attention as possible sources of stem cells to be used for clinical application in regenerative medicine. However, the biology of these cells and the signals required to effectively direct their differentiation are still largely unknown. This work was designed to define stemness properties in sheep amniotic epithelial cells (AECs) and evaluate their osteogenic potential in vitro. Sheep AECs express cell adhesion markers CD29 and CD49 and the pluripotent stem cell markers Oct4, SOX2, Nanog, and TERT that are typical of mesenchymal stem cells. Under specific inductive culture conditions, AECs undergo prompt osteogenic differentiation with extensive extracellular matrix mineralization, and the remarkable plasticity of these cells is progressively reduced after six expansion passages.[...]
Osteogenic potential of sheep amniotic epithelial stem cells
GLORIA, ALESSIA;TURRIANI, Maura;RUSSO, Valentina;MATTIOLI, Mauro
2012-01-01
Abstract
Amniotic membranes and amniotic fluid have attracted increased attention as possible sources of stem cells to be used for clinical application in regenerative medicine. However, the biology of these cells and the signals required to effectively direct their differentiation are still largely unknown. This work was designed to define stemness properties in sheep amniotic epithelial cells (AECs) and evaluate their osteogenic potential in vitro. Sheep AECs express cell adhesion markers CD29 and CD49 and the pluripotent stem cell markers Oct4, SOX2, Nanog, and TERT that are typical of mesenchymal stem cells. Under specific inductive culture conditions, AECs undergo prompt osteogenic differentiation with extensive extracellular matrix mineralization, and the remarkable plasticity of these cells is progressively reduced after six expansion passages.[...]I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
