Eicosanoids derive from the metabolism of arachidonic acid and serve as intracellular and extracellular signals, which are able to affect a wide variety of biologic processes, including inflammation.We summarize herein our data about 5-lipoxygenase (5-LOX) and cyclooxygenase-2 (COX-2) dependent enzymatic pathways in healthy and diseased porcine lungs. In detail, the following pulmonary diseases were investigated: Metastrongylus spp.-induced porcine parasitic bronchopneumonia, chronic enzootic pneumonia caused by Mycoplasma hyopneumoniae (Mh), acute pleuropneumonia by Actinobacillus pleuropneumoniae (App) and interstitial pneumonia caused by Porcine Circovirus type 2 (PCV2).Pulmonary tissue samples from healthy controls and diseased pigs were collected from slaughtered (healthy controls, Metastrongylus spp. and Mh-infected pigs) or spontaneously died (App and PCV2-infected pigs) animals, and adequately processed for histopathology, immunohistochemistry (IHC) and biochemical investigations.IHC was carried out by using polyclonal goat antibodies anti-5-LOX (C-19, Santa Cruz Biotechnology, 1 in 500) and anti-COX-2 (C-20, Santa Cruz Biotechnology, 1 in 250).5-LOX and COX-2 dependent enzymatic pathways were further investigated by Western blotting analysis and enzymatic activity assays. Arachidonic acid metabolites (namely leukotriene B4 and prostaglandin E2) were also quantified (Leukotriene B4 EIA kit™; Prostaglandin E2 EIA kit™; Cayman Chemical Company, USA).Our results confirm that 5-LOX and COX-2 are constitutively expressed in healthy porcine lungs. Furthermore, our data suggest that both investigated enzymatic pathways are significantly modified and/or over-expressed in porcine lungs affected by acute and chronic pneumonia caused by different etiological agents. Remarkably, 5-LOX- dependent biochemical pathway – which has been poorly investigated in veterinary medicine and is not inhibited by nonsteroidal anti-inflammatory drugs – seems to strongly participates in the acute and chronic inflammatory response in porcine pneumonia.[...]
Eicosanoids in Healthy and Diseased Porcine Lungs: Immunohistochemical and Biochemical Investigations.
MARRUCHELLA, GIUSEPPE;GIACOMINELLI STUFFLER, Roberto;
2012-01-01
Abstract
Eicosanoids derive from the metabolism of arachidonic acid and serve as intracellular and extracellular signals, which are able to affect a wide variety of biologic processes, including inflammation.We summarize herein our data about 5-lipoxygenase (5-LOX) and cyclooxygenase-2 (COX-2) dependent enzymatic pathways in healthy and diseased porcine lungs. In detail, the following pulmonary diseases were investigated: Metastrongylus spp.-induced porcine parasitic bronchopneumonia, chronic enzootic pneumonia caused by Mycoplasma hyopneumoniae (Mh), acute pleuropneumonia by Actinobacillus pleuropneumoniae (App) and interstitial pneumonia caused by Porcine Circovirus type 2 (PCV2).Pulmonary tissue samples from healthy controls and diseased pigs were collected from slaughtered (healthy controls, Metastrongylus spp. and Mh-infected pigs) or spontaneously died (App and PCV2-infected pigs) animals, and adequately processed for histopathology, immunohistochemistry (IHC) and biochemical investigations.IHC was carried out by using polyclonal goat antibodies anti-5-LOX (C-19, Santa Cruz Biotechnology, 1 in 500) and anti-COX-2 (C-20, Santa Cruz Biotechnology, 1 in 250).5-LOX and COX-2 dependent enzymatic pathways were further investigated by Western blotting analysis and enzymatic activity assays. Arachidonic acid metabolites (namely leukotriene B4 and prostaglandin E2) were also quantified (Leukotriene B4 EIA kit™; Prostaglandin E2 EIA kit™; Cayman Chemical Company, USA).Our results confirm that 5-LOX and COX-2 are constitutively expressed in healthy porcine lungs. Furthermore, our data suggest that both investigated enzymatic pathways are significantly modified and/or over-expressed in porcine lungs affected by acute and chronic pneumonia caused by different etiological agents. Remarkably, 5-LOX- dependent biochemical pathway – which has been poorly investigated in veterinary medicine and is not inhibited by nonsteroidal anti-inflammatory drugs – seems to strongly participates in the acute and chronic inflammatory response in porcine pneumonia.[...]I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
