The effect of the actin polymerization inhibitor cytochalasin D during in vitro capacitation of boar spermatozoa was assessed. In particular, the acquisition of fertilizing ability and the main intracellular signaling pathways were analyzed. It was found that treated spermatozoa were unable to carry out capacitation, even if the biochemical analyses of the signaling machineries failed to elucidate any biochemical alterations. Using a computational model based on the biological networks theory and representing the molecular interactions involved in male gamete post-ejaculatory life, it was possible to explain these data: actin polymerization interacted with different subcellular compartments, acting as a coordinator of molecular pathways involved in sperm cell function.
Role of Actin in Spermatozoa Function Through Biological Network Theory
BERNABO', NICOLA;LUCIDI, Pia;BERARDINELLI, Paolo
2012-01-01
Abstract
The effect of the actin polymerization inhibitor cytochalasin D during in vitro capacitation of boar spermatozoa was assessed. In particular, the acquisition of fertilizing ability and the main intracellular signaling pathways were analyzed. It was found that treated spermatozoa were unable to carry out capacitation, even if the biochemical analyses of the signaling machineries failed to elucidate any biochemical alterations. Using a computational model based on the biological networks theory and representing the molecular interactions involved in male gamete post-ejaculatory life, it was possible to explain these data: actin polymerization interacted with different subcellular compartments, acting as a coordinator of molecular pathways involved in sperm cell function.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
