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Organizzazioni amministrative e transizioni: le sfide del cambiamento

Impact of extraction methods on bioactive compounds and antioxidant activities of Thai jasmine rice leaf extracts

Studies have recently highlighted potential health benefits of young rice leaves; however, optimization of extraction process was often overlooked. The present study aimed to assess impacts of extraction methods, namely, maceration and decoction, on total phenolics content and antioxidant activities based on FRAP, DPPH, and ABTS assays of extracts from Thai cultivar of Jasmine rice leaves. The study examined influences of ethanol concentration (0–100% (v/v) for maceration), boiling time (10–3...

Extraction methods comparison and optimization for isoorientin and isovitexin from Thai jasmine rice leaves

Selected extraction techniques were first compared for young Jasmine rice leaves, which were evaluated as a novel source of flavone derivatives. Microwave-assisted extraction (MAE), which outperformed other tested methods in terms of phenolic content and antioxidant activity, was then evaluated and optimized. I-optimal design with response surface methodology was used to investigate the effects of and optimize MAE conditions in terms of the extraction time, solvent-to-solid ratio and MW power...

Peculiarity of porcine amniotic membrane and its derived cells: a contribution to the study of cell therapy from a large animal model

The aim of this work was to provide, for the first time, a protocol for isolation and characterization of stem cells from porcine amniotic membrane in view of their potential uses in regenerative medicine. From three samples of allanto-amnion recovered at delivery, the amniotic membrane was stripped from overlying allantois and digested with trypsin and collagenase to isolate epithelial (amniotic epithelial cells [AECs]) and mesenchymal cells, respectively. Proliferation, differentiation, and...

The impact of controlled ovarian stimulation hormones on the metabolic state and endocannabinoid system of human cumulus cells

Different Follicle Stimulating Hormone (FSH) formulation and Luteinizing Hormone (LH) are used in Assisted Reproductive Technology (ART) to induce follicles development and oocytes maturation, but it is still under debate which protocol is to be preferred. In the present study, the different effects on cumulus cells (CCs) of three controlled ovarian stimulation (COS) protocols, based on urinary FSH, recombinant FSH, or human Menopausal Gonadotropin (hMG) administration, were assessed. CCs wer...

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