Abnormal levels of Heat Shock Proteins (HSPs) have been observed in many human neoplasms,demonstrating both prognostic and therapeutic implications. Since human and canine osteosarcoma (OSA)share several biological and molecular features, the aim of this study was to evaluate theimmunohistochemical expression of Hsp27, Hsp72, Hsp73, Hsp90 in canine OSA model, in relation tohistological grade and overall survival (OS), as well as to expression of Hsp90 correlated molecules, suchas survivin and β-catenin, in order to investigate their potential prognostic and/or therapeutic value. A semiquantitativemethod was used to analyse results. Immunoreactivity of Hsp27, Hsp73 and Hsp90 was notassociated with histological grade or OS. However, widespread Hsp90 immunoreactivity detected in tumourcases and neoplastic emboli suggests that this protein can represent a potential molecular target for therapyof canine OSA. For Hsp72, immunosignal intensity and percentage of positive cells were highest (≥75%) ingrade III, whereas absent immunolabelling appeared to be associated with prolonged OS, thus suggesting apoor prognostic value of Hsp72 expression in canine OSA. Even though survivin nuclear immunosignalwas detected in all cases, it did not appear to be correlated to Hsp90 expression. On the other hand, acorrelation between the presence of β-catenin nuclear immunolabelling and a longer OS was found.[...]

Valutazione dell’espressione di heat shock proteins e pathway molecolari correlati nell’osteosarcoma canino (Expression of heat shock proteins and correlated molecular pathways in canine osteosarcoma).

ROMANUCCI, MARIARITA;BONGIOVANNI, LAURA;MALATESTA, DANIELA;PALMIERI, CHIARA;DELLA SALDA, Leonardo
2011-01-01

Abstract

Abnormal levels of Heat Shock Proteins (HSPs) have been observed in many human neoplasms,demonstrating both prognostic and therapeutic implications. Since human and canine osteosarcoma (OSA)share several biological and molecular features, the aim of this study was to evaluate theimmunohistochemical expression of Hsp27, Hsp72, Hsp73, Hsp90 in canine OSA model, in relation tohistological grade and overall survival (OS), as well as to expression of Hsp90 correlated molecules, suchas survivin and β-catenin, in order to investigate their potential prognostic and/or therapeutic value. A semiquantitativemethod was used to analyse results. Immunoreactivity of Hsp27, Hsp73 and Hsp90 was notassociated with histological grade or OS. However, widespread Hsp90 immunoreactivity detected in tumourcases and neoplastic emboli suggests that this protein can represent a potential molecular target for therapyof canine OSA. For Hsp72, immunosignal intensity and percentage of positive cells were highest (≥75%) ingrade III, whereas absent immunolabelling appeared to be associated with prolonged OS, thus suggesting apoor prognostic value of Hsp72 expression in canine OSA. Even though survivin nuclear immunosignalwas detected in all cases, it did not appear to be correlated to Hsp90 expression. On the other hand, acorrelation between the presence of β-catenin nuclear immunolabelling and a longer OS was found.[...]
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11575/8860
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