A rapid magnetoimmunosensor for the simultaneous determination of two cardiac biomarkers, amino-terminal pro-B-type natriuretic peptide (NT-proBNP) and C-reactive protein (CRP), in human serum is described. Specific capture antibodies were covalently immobilized onto carboxylic acid-modified magnetic beads. The quantification of NT-proBNP and CRP was performed by using indirect competitive and sandwich configurations, respectively, and horseradish peroxidase-labeled tracers. The use of dual screen-printed carbon electrodes allowed the achievement of simultaneous independent amperometric readout for each cardiac biomarker. The developed methodology showed very low limits of detection (0.47ngmL-1). An international standard for CRP serum spiked with NT-proBNP was analyzed to evaluate the usefulness of the magnetoimmunosensor.
Multiplexed determination of amino-terminal pro-B-type natriuretic peptide and C-reactive protein cardiac biomarkers in human serum at a disposable electrochemical magnetoimmunosensor
COMPAGNONE, DARIO;
2014-01-01
Abstract
A rapid magnetoimmunosensor for the simultaneous determination of two cardiac biomarkers, amino-terminal pro-B-type natriuretic peptide (NT-proBNP) and C-reactive protein (CRP), in human serum is described. Specific capture antibodies were covalently immobilized onto carboxylic acid-modified magnetic beads. The quantification of NT-proBNP and CRP was performed by using indirect competitive and sandwich configurations, respectively, and horseradish peroxidase-labeled tracers. The use of dual screen-printed carbon electrodes allowed the achievement of simultaneous independent amperometric readout for each cardiac biomarker. The developed methodology showed very low limits of detection (0.47ngmL-1). An international standard for CRP serum spiked with NT-proBNP was analyzed to evaluate the usefulness of the magnetoimmunosensor.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
