Malignant skin tumors with clear or vacuolated cytoplasm are uncommon in dog and, among them, clearcell adnexal carcinoma (CCAC) arises great interest for its variable differentiation, tough identification ofthe cell-of-origin and classification. This preliminary study involved 14 cases of CCAC, of whom 4 and 3analyzed by IHC(panCK, CK5, CK8-18, CALP, CK14, VIM, s100, NSE, GFAP, SMA, chrA, ki67,MHCII, p63) and TEM, respectively. Multilobular subcutaneous masses were characterized by polygonal,spindle, and variably vacuolated cells (PAS+, Oil Red O-), occasionally bi- and tri-nucleated, with raredermal papilla-like structures, squamous differentiation and keratinization. IHC revealed multifocalexpression of panCK and VIM, intermixed with areas negative for both markers and rare panCK+/VIM+cells, as well diffuse S100 expression. TEM confirmed the diagnosis of epithelial tumor characterized bysmall nuclei, marginated chromatin, 2-5μm in diameter vacuoles, containing proteinaceous granularmaterial and glycogen, rare haphazardly arranged cytoplasmic filaments (vimentin) and keratin bundles,desmosomes, rare round 0.3-0.4 μm in diameter single membrane-bound moderately electrondense granules(neuroendocrine-like), rare calcium deposits. These results suggest an adnexal origin of the tumors,specifically from a cell immunohistochemically similar to the bulge region and able to follow differentdegrees and lines of differentiation within the same lesion.[...]

Studio preliminare immunoistochimico ed ultrastrutturale del carcinoma cutaneo annessiale a cellule chiare del cane

PALMIERI, CHIARA;DELLA SALDA, Leonardo
2011-01-01

Abstract

Malignant skin tumors with clear or vacuolated cytoplasm are uncommon in dog and, among them, clearcell adnexal carcinoma (CCAC) arises great interest for its variable differentiation, tough identification ofthe cell-of-origin and classification. This preliminary study involved 14 cases of CCAC, of whom 4 and 3analyzed by IHC(panCK, CK5, CK8-18, CALP, CK14, VIM, s100, NSE, GFAP, SMA, chrA, ki67,MHCII, p63) and TEM, respectively. Multilobular subcutaneous masses were characterized by polygonal,spindle, and variably vacuolated cells (PAS+, Oil Red O-), occasionally bi- and tri-nucleated, with raredermal papilla-like structures, squamous differentiation and keratinization. IHC revealed multifocalexpression of panCK and VIM, intermixed with areas negative for both markers and rare panCK+/VIM+cells, as well diffuse S100 expression. TEM confirmed the diagnosis of epithelial tumor characterized bysmall nuclei, marginated chromatin, 2-5μm in diameter vacuoles, containing proteinaceous granularmaterial and glycogen, rare haphazardly arranged cytoplasmic filaments (vimentin) and keratin bundles,desmosomes, rare round 0.3-0.4 μm in diameter single membrane-bound moderately electrondense granules(neuroendocrine-like), rare calcium deposits. These results suggest an adnexal origin of the tumors,specifically from a cell immunohistochemically similar to the bulge region and able to follow differentdegrees and lines of differentiation within the same lesion.[...]
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11575/6171
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