Table olives are a very important component of the Mediterranean diet, but their production is still craft-based. The fermentation process depends on the indigenous microbiota, and it is often not standardized and difficult to be controlled. For this reason there is an increasing interest for starter culture development for olive fermentation, especially for lactic acid bacteria (LAB) since they play an important role for the safety and quality of the product.On the basis of the above considerations, our research was aimed at investigating the bacterial biodiversity table olives from different Italian cultivars. In particular, LAB were identified by a phenotypic and genetic approach based on 16S rRNA and recA gene sequencing and strains were typed by rep-PCR. To establish the molecular mechanisms that allow strain surviving in a stressful environment such as brine, a transposon mutagenesis was developed. In order to establish the randomness of the mutagenesis a Southern blot analysis was performed, which showed that the mutagenesis was random underling the efficiency of the developed strategy. A library of 6000 random transposon-mutants was generated. The development of an adapted screening strategy is now necessary to detect genes involved in the adaptation of Lactobacillus pentosus RT1 in brine. The obtained results should be useful for the selection of LAB to be used as starter cultures.[...]

Lactic acid bacteria in table olives: from identification to mutagenesis

PERPETUINI, GIORGIA;SCHIRONE, MARIA;TOFALO, ROSANNA;SUZZI, Giovanna;CORSETTI, Aldo
2012-01-01

Abstract

Table olives are a very important component of the Mediterranean diet, but their production is still craft-based. The fermentation process depends on the indigenous microbiota, and it is often not standardized and difficult to be controlled. For this reason there is an increasing interest for starter culture development for olive fermentation, especially for lactic acid bacteria (LAB) since they play an important role for the safety and quality of the product.On the basis of the above considerations, our research was aimed at investigating the bacterial biodiversity table olives from different Italian cultivars. In particular, LAB were identified by a phenotypic and genetic approach based on 16S rRNA and recA gene sequencing and strains were typed by rep-PCR. To establish the molecular mechanisms that allow strain surviving in a stressful environment such as brine, a transposon mutagenesis was developed. In order to establish the randomness of the mutagenesis a Southern blot analysis was performed, which showed that the mutagenesis was random underling the efficiency of the developed strategy. A library of 6000 random transposon-mutants was generated. The development of an adapted screening strategy is now necessary to detect genes involved in the adaptation of Lactobacillus pentosus RT1 in brine. The obtained results should be useful for the selection of LAB to be used as starter cultures.[...]
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11575/16839
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