Fourteen blood samples collected from clogs that were seropositive for Ehrlichia canis were examined For the presence of the citrate synthase gene using a highly specific and sensitive novel polymerase chain reaction assay. The assay detected E canis DNA in 3 dogs. The complete nucleotide sequence of the citrate synthase gene was determined in 2 of the test-positive samples, and represents the first sequence of the gene to be derived from Italian isolates. The sequence data displayed high identity (99.2%) between the geographically separated Italian samples and the Oklahoma strain of E canis. The high-sequence conservation revealed by molecular analysis confirmed the usefulness of the citrate synthase gene as a target for detection of E canis[...]
Direct identification of Ehrlichia canis by a polymerase chain reaction method and molecular analysis of the citrate sinthase gene from various italian strains
MARSILIO, Fulvio;DI MARTINO, Barbara;
2006-01-01
Abstract
Fourteen blood samples collected from clogs that were seropositive for Ehrlichia canis were examined For the presence of the citrate synthase gene using a highly specific and sensitive novel polymerase chain reaction assay. The assay detected E canis DNA in 3 dogs. The complete nucleotide sequence of the citrate synthase gene was determined in 2 of the test-positive samples, and represents the first sequence of the gene to be derived from Italian isolates. The sequence data displayed high identity (99.2%) between the geographically separated Italian samples and the Oklahoma strain of E canis. The high-sequence conservation revealed by molecular analysis confirmed the usefulness of the citrate synthase gene as a target for detection of E canis[...]I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
