Nucleus transfer in mammals:noninvasive approaches for thepreparation of cytoplastsJosef Fulka Jr1, Pasqualino Loi2, Helena Fulka1, Grazyna Ptak2 and Taku Nagai31Institute of Animal Production, POB 1, Prague 10, Czech Republic2University of Teramo, Department of Comparative Biomedical Sciences, Teramo 64100, Italy3National Institute of Livestock and Grassland Science, Tsukuba, Ibaraki 305-0901, JapanThe production of cloned animals is a difficult and complexprocedure that requires two basic steps. First, thecytoplast must be prepared by the enucleation of metaphaseII oocytes. Second, the nucleus is transferredeither by fusion or by direct microinjection into the cytoplast.The preparation of cytoplasts is a crucial stepbecause they must be able to reprogram the transferrednucleus and to secure the development of reconstructedembryos. Moreover, the classicalmechanical enucleationof metaphase II oocytes is rather technically difficult,requiring good equipment and considerable micromanipulationskill. For this reason the simplification of thisstep is permanently in the centre of interest of thosescientists who are involved in the production of clones.[...]
Nucleus transfer in mammals: noninvasive approaches for the preparation of cytoplasts
LOI, PasqualinoMembro del Collaboration Group
;PTAK, GrazynaMembro del Collaboration Group
;
2004-01-01
Abstract
Nucleus transfer in mammals:noninvasive approaches for thepreparation of cytoplastsJosef Fulka Jr1, Pasqualino Loi2, Helena Fulka1, Grazyna Ptak2 and Taku Nagai31Institute of Animal Production, POB 1, Prague 10, Czech Republic2University of Teramo, Department of Comparative Biomedical Sciences, Teramo 64100, Italy3National Institute of Livestock and Grassland Science, Tsukuba, Ibaraki 305-0901, JapanThe production of cloned animals is a difficult and complexprocedure that requires two basic steps. First, thecytoplast must be prepared by the enucleation of metaphaseII oocytes. Second, the nucleus is transferredeither by fusion or by direct microinjection into the cytoplast.The preparation of cytoplasts is a crucial stepbecause they must be able to reprogram the transferrednucleus and to secure the development of reconstructedembryos. Moreover, the classicalmechanical enucleationof metaphase II oocytes is rather technically difficult,requiring good equipment and considerable micromanipulationskill. For this reason the simplification of thisstep is permanently in the centre of interest of thosescientists who are involved in the production of clones.[...]I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
