Evaluation of an handheld electrochemical Comparison of breath hydrogen concentration in dog administration of 5 and 4 sugar solution

The purpose of this project is to evaluate the hydrogen breath concentration after oral administration of 3 different solutions of 5 (solution 1: 4.0 g xylose, 16g lactulose, 4.0 g rhamnose, 2.0 g 3-0-methylglucose and 4.0g sucrose), 2 (solution 2: 4.0 g lactulose and 4.0g L-rhamnose ), and 4 (solution 3: 4.0 g xylose, 16 g lactulose, 4.0 g rhamnose, 2.0 g 3-0-methylglucose) sugars, in clinically healthy dogs. Eight clinically healthy female dogs were fed with a commercial diet. Food was withdrawn for 12 hours before testing the hydrogen breath test. A baseline breath sample was collected before the oral administration of the sugar solutions. The samples were collected every 15, and 30 minutes for 8 hours. Mean  SD AUC for test 2, 5, and 4 inert sugars solution was 54.48  26.66, 72.14 29.00 , and 47.05  20.49 ppm /h., respectively. After administration of solution1, breath H2 excretion began to be significantly higher (P< 0.02) than fasting value at 75’. A maximum mean breath H2 concentration of 14.82  7.42 was observed at 2.25 h. After administration of solution2 breath excretion was significantly higher than fasting at 90’ (P< 0.029). A maximum mean breath test of 11.2  4.30 yield at 2 hr. No significantly differences was observed between fasting and mean excretion H2 after administration of solution 4. Although further investigations are required, we can conclude that solution 1 shows an higher meaning for the breath H2 excretion than the other two solutions.